Transgenic engineering of male-specific muscular hypertrophy

被引：27

作者：

Pirottin, D

Grobet, L

Adamantidis, A

Farnir, F

Herens, C

Schroder, HD

Georges, M

机构：

[1] Univ Liege B43, Fac Vet Med, Dept Anim Prod, Unit Factorial & Mol Genet, B-4000 Liege, Belgium

[2] Univ Liege B43, Fac Vet Med, Dept Pathol & Morphol, Unit Embryol, B-4000 Liege, Belgium

[3] Univ Liege, Res Ctr Cellular & Mol Neurobiol, B-4020 Liege, Belgium

[4] Univ Liege, CHU, Ctr Human Genet, B-4000 Liege, Belgium

[5] Odense Univ Hosp, Inst Pathol, DK-5000 Odense, Denmark

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 18期

关键词：

gene targeting; myostatin; Y chromosome;

D O I：

10.1073/pnas.0502426102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency-associated myostatin propeptide under control of the myosin light chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass. This experiment demonstrates the feasibility of a more efficient cattle production system combining superior beef production abilities for bulls and dairy abilities for cows.

引用

页码：6413 / 6418

页数：6

共 27 条

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