On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes

被引：20

作者：

Kjellström, S

Emnéus, J

Laurell, T

Heintz, L

Marko-Varga, G

机构：

[1] Univ Lund, Dept Analyt Chem, S-22100 Lund, Sweden

[2] Univ Lund, Lund Inst Technol, Dept Elect Measurements, S-22100 Lund, Sweden

[3] Astro Draco AB, Bioanalyt Chem, Preclin Res & Dev, S-22100 Lund, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 823卷 / 1-2期

关键词：

sample handling; microdialysis; leukotrienes; eicosanoids;

D O I：

10.1016/S0021-9673(98)00361-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perfusion liquid used for sampling hydrophobic eicosanoids in biological systems was also studied. The total time for one analysis was 7.6 min allowing seven measurements per hour for monitoring kinetic changes in biological systems. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：489 / 496

页数：8

共 24 条

[1] STEADY-STATE THEORY FOR QUANTITATIVE MICRODIALYSIS OF SOLUTES AND WATER INVIVO AND INVITRO [J].