Isolation and characterization of xylans from seed pericarp of Argania spinosa fruit

被引:54
作者
Habibi, Y [1 ]
Vignon, MR [1 ]
机构
[1] Univ Grenoble 1, CNRS, Ctr Rech Macromol Vegetales, F-38041 Grenoble, France
关键词
Argania spinosa; seed; (1 -> 4)-beta-D-xylan; H-1 and C-13 NMR spectroscopy;
D O I
10.1016/j.carres.2005.01.039
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hemicellulose-type polysaccharides were isolated from the pericarp of seeds of Argania spinosa (L.) Skeels fruit by sequential alkaline extractions and fractionated by precipitation. Water soluble and water insoluble fractions were obtained, purified and characterized by sugar analysis and H-1 and C-13 NMR spectroscopy. The water soluble fractions were assumed to be (4-O-methyl-D-glucturono)-D-xylans. with 4-O-methyl-D-glucopyranosyluronic acid groups linked to C-2 of a (1 -> 4)-beta-D-xylan. The 1H NMR spectrum showed that the water soluble xylans have, on average, one non-reducing terminal residue of 4-O-methyl-D-glucuronic acid for every seven xylose units. The water insoluble fractions consisted of a neutral xylan with linear (1 -> 4)-beta-D-xylopyranosyl units. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1431 / 1436
页数:6
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