Engineering sulfotransferases to modify heparan sulfate

被引：63

作者：

Xu, Ding ^{[1
]}

Moon, Andrea F. ^{[2
]}

Song, Danyin ^{[1
]}

Pedersen, Lars C. ^{[2
]}

Liu, Jian ^{[1
]}

机构：

[1] Univ N Carolina, Sch Pharm, Div Med Chem & Nat Prod, Chapel Hill, NC 27599 USA

[2] Natl Inst Environm Hlth Sci, NIH, Struct Biol Lab, Res Triangle Pk, NC 27709 USA

来源：

NATURE CHEMICAL BIOLOGY | 2008年 / 4卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nchembio.66

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthesis of heparan sulfate (HS) involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations of these residues altered the substrate specificities. Our results demonstrate the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions.

引用

页码：200 / 202

页数：3

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