Fatty acyl pheromone analogue-containing lipids and their roles in sex pheromone biosynthesis in the lightbrown apple moth, Epipyhas postvittana (Walker)

被引:15
作者
Foster, SP [1 ]
机构
[1] Hort & Food Res Inst New Zealand Ltd, Mt Albert Res Ctr, Auckland, New Zealand
关键词
PBAN; regulation; fatty acid CoA esters; fatty acid metabolism; triglycerides;
D O I
10.1016/S0022-1910(00)00139-6
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The pheromone gland of the moth Epiphyas postvittana was analysed for lipids containing the fatty acyl pheromone analogue (FAPA) of the component, (E)-11-tetradecenyl acetate. The FAPA was found predominantly in the triglycerides (TGs), and to a lesser extent in the choline phosphatides. The FAPA was found to be exclusively on the sn-1 or sn-3 position (probably the latter) of the TGs. When pheromone gland lipid extracts were eluted through silica solid phase extraction, a significant proportion of the FAPA was not recovered. Changes in titre of this non-recoverable FAPA paralleled changes in pheromone titre in females. In contrast, changes in recoverable FAPA (mostly in the TGs) titre showed a gradual increase with time after eclosion. The properties of this non-recoverable FAPA were consistent with it being the CoA ester of the FAPA. Thus, it appears that the FAPA-CoA ester is the immediate lipid precursor of the pheromone, and that the FAPA-containing TGs are formed by reaction of the FAPA-CoA with 1,2-DGs, as a consequence of the rate-limiting reduction of the FAPA-CoA. Finally, injection of PBAN into females decapitated for 3 days resulted in a decrease in recoverable FAPA and an increase in non-recoverable FAPA, suggesting that PBAN influences the lipolysis of TGs. Overall these data suggest that there are two routes for biosynthesis of the pheromone component E11-14:OAc in E. postvittana: a de novo route, directly via the CoA esters of the various fatty acid intermediates, and a less direct route via the lipolysis of FAPA-containing TGs. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
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页码:433 / 443
页数:11
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