Acute and chronic leptin effect upon in vivo and in vitro rat thyroid iodide uptake

Leptin has stimulatory effects on the hypothalamic-pituitary-thyroid axis and on deiodinases activities. Here, we evaluated the effect of leptin injection upon in vivo and in vitro thyroid 1251 uptake (RAIU). We designed two experiments: acute leptin (LepA) with a single dose of leptin (8 mu g/100 g BW/sc), and chronic leptin (LepC, injected with the same dose of LepA, once a day, for 6 days. In parallel, control groups were saline-injected. For in vivo study, part of the animals were injected with (125)1 (3700 Bq) and killed after 15 or 120 min. In vivo thyroid RAIU was not changed in LepA animals. However, LepC animals showed higher in vivo thyroid RAIU (15 min:+130% and 120 min:+72%;p<0.05). For in vitro study, the other animals were killed and their thyroids were incubated with I-125. Thyroids explants from LepA and LepC groups presented lower thyroid 125, content (-32% and -29% p<0.05, respectively). The amount of our data suggest that, in vitro, leptin causes a direct inhibition of the rat thyroid RAIU, but in vivo, the effect of leptin was different according to the treatment period, which indicates that other indirect mechanisms are involved in the in vivo leptin chronic stimulation of the thyroid gland. (C) 2007 Elsevier Inc. All rights reserved.