A proteomics approach to identify changes in protein profiles in pre-cancerous colon

被引：18

作者：

Drew, JE ^{[1
]}

Rucklidge, GJ

Duncan, G

Lufty, A

Farquharson, AJ

Reid, MD

Russell, WR

Morrice, PC

Arthur, JR

Duthie, GG

机构：

[1] Rowett Res Inst, Cellular Integr Div, Aberdeen AB21 9SB, Scotland

[2] Rowett Res Inst, Proteom Unit, Aberdeen AB21 9SB, Scotland

[3] Dumfries Royal Infirm, Dumfries, Scotland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 330卷 / 01期

关键词：

proteomics; colon cancer; rat; aberrant crypt foci; prostaglandin;

D O I：

10.1016/j.bbrc.2005.02.125

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of colon cancer is characterised by alterations in multiple genetic and epigenetic pathways in colon tissue leading ultimately to deregulation of colon epithelial cells. Early detection is an important factor in decreasing colon cancer deaths. Proteomic techniques were used to identify potential early markers in colon tissue exhibiting pre-cancerous activity that may characterise pathological changes in a chemically induced colon cancer rat model. Protein profiles were assessed in soluble and insoluble fractions prepared from distal colon of rats treated with the colonotropic carcinogen, dimethylhydrazine. Alterations in protein profiles were associated with the presence of aberrant crypt foci, hyperplasia and dysplasia, microanatomical changes, and metabolic changes in rat colon. These changes may have a potential role in the identification of pre-pathological features preceding colon tumorigenesis. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：81 / 87

页数：7

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