Characterization of typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the classical O55 serogroup by RAPD analysis

被引:6
作者
Girao, DM [1 ]
Bando, SY [1 ]
Girao, VBD [1 ]
Moreira, CA [1 ]
Fracalanzza, SEL [1 ]
Trabulsi, LR [1 ]
Monteiro-Neto, V [1 ]
机构
[1] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Rio De Janeiro, Brazil
来源
REVISTA DE MICROBIOLOGIA | 1999年 / 30卷 / 04期
关键词
enteropathogenic Escherichia coli; EPEC; genetic diversity; RAPD analysis;
D O I
10.1590/S0001-37141999000400013
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genetic diversity of 41 typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the serogroup O55 was analyzed by using the random amplified polymorphic DNA (RAPD) method. All typical EPEC O55 strains were grouped in two clusters (A and C) and belonged to the serotype O55:H6, while cluster B included all atypical strains, which were of the serotype O55:H7. The three groups also included non-motile strains. RAPD may be a useful method for epidemiological studies on E. coli O55 infection.
引用
收藏
页码:365 / 368
页数:4
相关论文
共 11 条
[1]   Characterization of enteroinvasive Escherichia coli and Shigella strains by RAPD analysis [J].
Bando, SY ;
do Valle, GRF ;
Martinez, MB ;
Trabulsi, LR ;
Moreira-Filho, CA .
FEMS MICROBIOLOGY LETTERS, 1998, 165 (01) :159-165
[2]   ESCHERICHIA-COLI SEROGROUP O111 INCLUDES SEVERAL CLONES OF DIARRHEAGENIC STRAINS WITH DIFFERENT VIRULENCE PROPERTIES [J].
CAMPOS, LC ;
WHITTAM, TS ;
GOMES, TAT ;
ANDRADE, JRC ;
TRABULSI, LR .
INFECTION AND IMMUNITY, 1994, 62 (08) :3282-3288
[3]  
Goncalves AG, 1997, INFECT IMMUN, V65, P2034
[4]  
Kaper JB, 1996, REV MICROBIOL, V27, P130
[5]   VERO RESPONSE TO A CYTOTOXIN OF ESCHERICHIA-COLI [J].
KONOWALCHUK, J ;
SPEIRS, JI ;
STAVRIC, S .
INFECTION AND IMMUNITY, 1977, 18 (03) :775-779
[6]   THE DIARRHEAL RESPONSE OF HUMANS TO SOME CLASSIC SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA-COLI IS DEPENDENT ON A PLASMID ENCODING AN ENTEROADHESIVENESS FACTOR [J].
LEVINE, MM ;
NATARO, JP ;
KARCH, H ;
BALDINI, MM ;
KAPER, JB ;
BLACK, RE ;
CLEMENTS, ML ;
OBRIEN, AD .
JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (03) :550-559
[7]  
Nataro JP, 1998, CLIN MICROBIOL REV, V11, P142
[8]   Virulence properties of atypical EPEC strains [J].
Pelayo, JS ;
Scaletsky, ICA ;
Pedroso, MZ ;
Sperandio, V ;
Girón, JA ;
Frankel, G ;
Trabulsi, LR .
JOURNAL OF MEDICAL MICROBIOLOGY, 1999, 48 (01) :41-49
[9]   Clonal structure and virulence factors in strains of Escherichia coli of the classic serogroup O55 [J].
Rodrigues, J ;
Scaletsky, ICA ;
Campos, LC ;
Gomes, TAT ;
Whittam, TS ;
Trabulsi, LR .
INFECTION AND IMMUNITY, 1996, 64 (07) :2680-2686
[10]   Use of gene probes and adhesion tests to characterise Escherichia coli belonging to enteropathogenic serogroups isolated in the United Kingdom [J].
Scotland, SM ;
Smith, HR ;
Cheasty, T ;
Said, B ;
Willshaw, GA ;
Stokes, N ;
Rowe, B .
JOURNAL OF MEDICAL MICROBIOLOGY, 1996, 44 (06) :438-443