Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR

被引：83

作者：

Wadowsky, RM

Laus, S

Green, M

Webber, SA

Rowe, D

机构：

[1] Childrens Hosp Pittsburgh, Dept Pathol, Pittsburgh, PA 15213 USA

[2] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Infect Dis & Microbiol, Pittsburgh, PA 15213 USA

[3] Univ Pittsburgh, Sch Med, Dept Pathol, Pittsburgh, PA 15213 USA

[4] Univ Pittsburgh, Sch Med, Dept Pediat, Pittsburgh, PA 15213 USA

[5] Univ Pittsburgh, Sch Med, Dept Surg, Pittsburgh, PA 15213 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 11期

关键词：

D O I：

10.1128/JCM.41.11.5245-5249.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood (n = 60) and plasma (n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) (n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly (r(2) > 0.900), whereas the plasma and PBL loads correlated poorly (r(2) = 0.512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load.

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页码：5245 / 5249

页数：5

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