HPLC determination of ochratoxin a in a low volume of human blood serum

被引：14

作者：

Ghali, R. ^{[1
]}

Hmaissia-Khlifa, K. ^{[2
]}

Mezigh, Ch. ^{[2
]}

Ghorbel, H. ^{[1
]}

Maaroufi, K. ^{[3
]}

Maschgoul, S. ^{[2
]}

Hedilli, A. ^{[1
]}

机构：

[1] Ctr Urgent Med Aid Tunis, Res Unit Toxicol & Environm, Lab Biol & Toxicol, Tunis 1008, Tunisia

[2] Mil Hosp Tunis, Tunis, Tunisia

[3] Fac Pharm Monastir, Monastir, Tunisia

来源：

ANALYTICAL LETTERS | 2008年 / 41卷 / 05期

关键词：

ochratoxin A; serum; HPLC; SPE; Tunisia;

D O I：

10.1080/00032710801934528

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatography (HPLC) method has been developed for the determination of ochratoxin A (OTA) in human blood serum. Samples were purified on a C18 solid phase extraction column. The developed method required a relatively low serum volume (0.5ml). Significant correlation (r of 0.998) was found over the range from 0.10 to 8ng/ml, with a detection limit of 0.1ng/ml and better performance in terms of precision and accuracy. Mean recoveries at 0.5 and 2ng/ml were respectively 69.7 +/- 1.2 and 71.9 +/- 2.8%. This method was used as a rapid and noninvasive tool to assess human exposure to OTA. Among 40 analyzed serum samples, 27.5% were found to contain OTA with levels going from 0.1 to 11.98ng/ml with a mean concentration of 0.73 +/- 2.35ng/ml.

引用

页码：757 / 766

页数：10

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