Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4

被引：1377

作者：

Peled, A

Petit, I

Kollet, O

Magid, M

Ponomaryov, T

Byk, T

Nagler, A

Ben-Hur, H

Many, A

Shultz, L

Lider, O

Alon, R

Zipori, D

Lapidot, T ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Immunol, IL-76100 Rehovot, Israel

[2] Weizmann Inst Sci, Dept Mol Cell Biol, IL-76100 Rehovot, Israel

[3] Hadassah Univ Hosp, IL-91120 Jerusalem, Israel

[4] Sourasky Med Ctr, IL-64239 Tel Aviv, Israel

[5] Jackson Lab, Bar Harbor, ME 04609 USA

来源：

SCIENCE | 1999年 / 283卷 / 5403期

关键词：

D O I：

10.1126/science.283.5403.845

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Stem cell homing and repopulation are not well understood. The chemokine stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 were found to be critical for murine bone marrow engraftment by human severe combined immunodeficient (SCID) repopulating stem cells. Treatment of human cells with antibodies to CXCR4 prevented engraftment. In vitro CXCR4-dependent migration to SDF-1 of CD34(+)CD38(-/low) cells correlated with in vivo engraftment and stem cell function. Stem cell factor and interleukin-6 induced CXCR4 expression on CD34(+) cells, which potentiated migration to SDF-1 and engraftment in primary and secondary transplanted mice. Thus, up-regulation of CXCR4 expression may be useful for improving engraftment of repopulating stem cells in clinical transplantation.

引用

页码：845 / 848

页数：4

共 20 条

[1] The chemokine SDF-1 is a chemoattractant for human CD34(+) hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34(+) progenitors to peripheral blood
Aiuti, A
Webb, IJ
Bleul, C
Springer, T
GutierrezRamos, JC
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 185 (01) : 111 - 120
[2] A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1)
Bleul, CC
Fuhlbrigge, RC
Casasnovas, JM
Aiuti, A
Springer, TA
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (03) : 1101 - 1109
[3] Kinetic evidence of the regeneration of multilineage hematopoiesis from primitive cells in normal human bone marrow transplanted into immunodeficient mice
Cashman, JD
Lapidot, T
Wang, JCY
Doedens, M
Shultz, LD
Lansdorp, P
Dick, JE
Eaves, CJ
[J]. BLOOD, 1997, 89 (12) : 4307 - 4316
[4] Christianson SW, 1997, J IMMUNOL, V158, P3578
[5] Sustained, retransplantable, multilineage engraftment of highly purified adult human bone marrow stem cells in vivo
Civin, CI
AlmeidaPorada, G
Lee, MJ
Olweus, J
Terstappen, LWMM
Zanjani, ED
[J]. BLOOD, 1996, 88 (11) : 4102 - 4109
[6] Expression of the human immunodeficiency virus type-1 coreceptors CXCR-4 (fusin, LESTR) and CKR-5 in CD34(+) hematopoietic progenitor cells
Deichmann, M
Kronenwett, R
Haas, R
[J]. BLOOD, 1997, 89 (10) : 3522 - 3528
[7] In vitro behavior of hematopoietic progenitor cells under the influence of chemoattractants: Stromal cell-derived factor-1, steel factor, and the bone marrow environment
Kim, CH
Broxmeyer, HE
[J]. BLOOD, 1998, 91 (01) : 100 - 110
[8] CYTOKINE STIMULATION OF MULTILINEAGE HEMATOPOIESIS FROM IMMATURE HUMAN-CELLS ENGRAFTED IN SCID MICE
LAPIDOT, T
PFLUMIO, F
DOEDENS, M
MURDOCH, B
WILLIAMS, DE
DICK, JE
[J]. SCIENCE, 1992, 255 (5048) : 1137 - 1141
[9] Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: Implications for gene therapy
Larochelle, A
Vormoor, J
Hanenberg, H
Wang, JCY
Bhatia, M
Lapidot, T
Moritz, T
Murdoch, B
Xiang, LX
Kato, I
Williams, DA
Dick, JE
[J]. NATURE MEDICINE, 1996, 2 (12) : 1329 - 1337
[10] THE SCID-HU MOUSE - MURINE MODEL FOR THE ANALYSIS OF HUMAN HEMATOLYMPHOID DIFFERENTIATION AND FUNCTION
MCCUNE, JM
NAMIKAWA, R
KANESHIMA, H
SHULTZ, LD
LIEBERMAN, M
WEISSMAN, IL
[J]. SCIENCE, 1988, 241 (4873) : 1632 - 1639

← 1 2 →