Convergence of developmental mutants into a single tomato model system: 'Micro-Tom' as an effective toolkit for plant development research

被引:137
作者
Carvalho, Rogerio F. [1 ]
Campos, Marcelo L. [1 ]
Pino, Lilian E. [1 ,2 ]
Crestana, Simone L. [1 ]
Zsoegoen, Agustin [1 ]
Lima, Joni E. [1 ,2 ]
Benedito, Vagner A. [3 ]
Peres, Lazaro E. P. [1 ]
机构
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Biol Sci LCB, Lab Hormonal Control Plant Dev, BR-13418900 Piracicaba, SP, Brazil
[2] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13400970 Piracicaba, SP, Brazil
[3] W Virginia Univ, Genet & Dev Biol Program, Div Plant & Soil Sci, Morgantown, WV 26506 USA
基金
巴西圣保罗研究基金会;
关键词
hormonal mutants; Solanum lycopersicum; model organism; photomorphogenesis; plant development; SOLANUM-LYCOPERSICON; IN-VITRO; SIGNAL-TRANSDUCTION; ETHYLENE PERCEPTION; PROCERA MUTANT; ABSCISIC-ACID; SEED DORMANCY; FRUIT-SET; WILD-TYPE; LIGHT;
D O I
10.1186/1746-4811-7-18
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably compare phenotypes. The lack of tomato developmental mutants in a single genetic background prevents the stacking of mutations to facilitate analysis of double and multiple mutants, often required for elucidating developmental pathways. Results: We took advantage of the small size and rapid life cycle of the tomato cultivar Micro-Tom (MT) to create near-isogenic lines (NILs) by introgressing a suite of hormonal and photomorphogenetic mutations (altered sensitivity or endogenous levels of auxin, ethylene, abscisic acid, gibberellin, brassinosteroid, and light response) into this genetic background. To demonstrate the usefulness of this collection, we compared developmental traits between the produced NILs. All expected mutant phenotypes were expressed in the NILs. We also created NILs harboring the wild type alleles for dwarf, self-pruning and uniform fruit, which are mutations characteristic of MT. This amplified both the applications of the mutant collection presented here and of MT as a genetic model system. Conclusions: The community resource presented here is a useful toolkit for plant research, particularly for future studies in plant development, which will require the simultaneous observation of the effect of various hormones, signaling pathways and crosstalk.
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页数:14
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