High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein

被引：43

作者：

Maulucci, Giuseppe ^{[2
]}

Labate, Valentina ^{[1
]}

Mele, Marina ^{[1
]}

Panieri, Emiliano ^{[1
]}

Arcovito, Giuseppe ^{[2
]}

Galeotti, Tommaso ^{[1
]}

Ostergaard, Henrik ^{[3
]}

Winther, Jakob R. ^{[4
]}

De Spirito, Marco ^{[2
]}

Pani, Giovambattista ^{[1
]}

机构：

[1] Catholic Univ, Sch Med, Inst Gen Pathol, I-00168 Rome, Italy

[2] Catholic Univ, Sch Med, Inst Phys, I-00168 Rome, Italy

[3] Novo Nordisk AS, DK-2760 Malov, Denmark

[4] Dept Mol Biol, DK-2100 Copenhagen, Denmark

来源：

SCIENCE SIGNALING | 2008年 / 1卷 / 43期

关键词：

D O I：

10.1126/scisignal.143pl3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time "redox maps" of adherent cells and to score subtle changes in the intracellular redox state, such as those induced by overexpression of redox-active proteins. This strategy for in vivo imaging of redox signaling circumvents many of the technical limitations currently encountered in the study of complex redox-based phenomena and promises to contribute substantially to this expanding area of signal transduction.

引用

页数：15

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