Immobilization of glucose oxidase on films of 3-methylthiophene/thiophene-3-acetic acid copolymer and its application to a sensor

被引：13

作者：

Kuwahara, T ^{[1
]}

Oshima, K ^{[1
]}

Yamauchi, T ^{[1
]}

Shimomura, M ^{[1
]}

Miyauchi, S ^{[1
]}

机构：

[1] Nagaoka Univ Technol, Fac Engn, Dept Bioengn, Nagaoka, Niigata 9402188, Japan

来源：

KOBUNSHI RONBUNSHU | 2004年 / 61卷 / 02期

关键词：

glucose sensor; glucose oxidase; immobilization; copolymer; electrochemical polymerization;

D O I：

10.1295/koron.61.122

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

3-Methylthiophene/thiophene-3-acetic acid (3MT/T3A) copolymer films were fabricated by electrochemical polymerization and glucose oxidase (GOD) was immobilized covalently on the films. Conductivity of the copolymer films was found to increase with decreasing content of T3A units. An almost constant amount of GOD was immobilized on the copolymer films whose contents of T3A units were larger than 30%. The activity of immobilized GOD was not affected remarkably by 3MT/T3A ratio in the copolymer films used. Amperometric sensing of glucose with the GOD-immobilized copolymer films gave the result that large response currents were observed by use of the copolymer films with a low content of T3A units. The result suggests that the sensitivity depends on the conductivity of the copolymer films as well as the activity of immobilized GOD.

引用

页码：122 / 126

页数：5

共 20 条

[1] Photovoltaic cells with a conjugated polyelectrolyte [J].

Ding, L ;

Jonforsen, M ;

Roman, LS ;

Andersson, MR ;

Inganäs, O .

SYNTHETIC METALS, 2000, 110 (02) :133-140

[2] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF A DEGLYCOSYLATED GLUCOSE-OXIDASE FROM PENICILLIUM-AMAGASAKIENSE [J].

HENDLE, J ;

HECHT, HJ ;

KALISZ, HM ;

SCHMID, RD ;

SCHOMBURG, D .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (04) :1167-1169

[3]

Ju HX, 1998, ELECTROANAL, V10, P541, DOI 10.1002/(SICI)1521-4109(199807)10:8<541::AID-ELAN541>3.0.CO

[4]

2-J

[5] Titration behaviors and spectral properties of hydrophobically modified water-soluble polythiophenes [J].

Kim, BS ;

Fukuoka, H ;

Gong, HP ;

Osada, Y .

EUROPEAN POLYMER JOURNAL, 2001, 37 (12) :2499-2503

[6] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF PEROXIDASE FROM A FUNGUS ARTHROMYCES-RAMOSUS [J].

KUNISHIMA, N ;

FUKUYAMA, K ;

WAKABAYASHI, S ;

SUMIDA, M ;

TAKAYA, M ;

SHIBANO, Y ;

AMACHI, T ;

MATSUBARA, H .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1993, 15 (02) :216-220

[7] Electrochemically pH-controllable 3-substituted polythiophene films [J].

Li, JQ ;

Aoki, K .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1998, 458 (1-2) :155-160

[8] Synthesis and properties of poly(3-thiopheneacetic acid) and its networks via electropolymerization [J].

Liaw, DJ ;

Liaw, BY ;

Gong, JP ;

Osada, Y .

SYNTHETIC METALS, 1999, 99 (01) :53-59

[9] SOLVENT CONTENT OF PROTEIN CRYSTALS [J].

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+

[10] Amperometric biosensors for sensing of hydrogen peroxide based on electron transfer between horseradish peroxidase and ferrocene as a mediator [J].

Nakabayashi, Y ;

Yoshikawa, H .

ANALYTICAL SCIENCES, 2000, 16 (06) :609-613

← 1 2 →