Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L.

被引：74

作者：

Avila, CM

Sillero, JC

Rubiales, D

Moreno, MT

Torres, AM

机构：

[1] CIFA Alameda Obispo, Dept Mejora & Agron, Cordoba 14080, Spain

[2] CSIC, Inst Agr Sostenible, Dept Agron & Mejora Genet Vegetal, Cordoba 14080, Spain

来源：

THEORETICAL AND APPLIED GENETICS | 2003年 / 107卷 / 02期

关键词：

Vicia faba; rust; Uromyces viciae-fabae; resistance genes; marker-assisted selection;

D O I：

10.1007/s00122-003-1254-8

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 [作物学];

摘要：

Bulk segregant analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a gene determining hypersensitive resistance in Vicia faba line 2N52 against race 1 of the rust fungus Uromyces viciae-fabae. The monogenic nature of the resistance was determined by analyzing the F-2 population from a cross between resistant line 2N52 and susceptible line VF-176, and further confirmed in the F-2:3-derived families. Linkage of the RAPD markers was confirmed by screening 55 F-2 plants segregating for resistance. Three RAPD markers (OPD13(736), OPL18(1032) and OPI20(900)) were mapped in coupling phase to the resistance gene for race 1 (Uvf-1). No recombinants between OPI20(900) and Uvf-1 were detected. Two additional markers (OPP02(1172) and OPR07(930)) were linked to the gene in repulsion phase at a distance of 9.9 and 11.5 cM, respectively. The application of marker-assisted selection to develop new faba bean varieties with rust resistance genes is discussed.

引用

页码：353 / 358

页数：6

共 39 条

[1]

Improved selection with newly identified RAPD markers linked to resistance gene to four pathotypes of Colletotrichum lindemuthianum in common bean [J].