Size heterogeneity in the 3' noncoding region of south American isolates of yellow fever virus

被引:41
作者
Bryant, JE
Vasconcelos, PFC
Rijnbrand, RCA
Mutebi, JP
Higgs, SIH
Barrett, ADT [1 ]
机构
[1] Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA
[2] Univ Texas, Med Branch, Ctr Biodef & Emerging Infect Dis, Galveston, TX 77555 USA
[3] Univ Texas, Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA
[4] Minist Hlth, Inst Evandro Chagas, Dept Abovirol, Belem, Para, Brazil
[5] Chicago Dept Publ Hlth, Chicago, IL USA
关键词
D O I
10.1128/JVI.79.6.3807-3821.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The 3' noncoding region (3' NCR) of flaviviruses contains secondary and tertiary structures essential for virus replication. Previous studies of yellow fever virus (YFV) and dengue virus have found that modifications to the 3' NCR are sometimes associated with attenuation in vertebrate and/or mosquito hosts. The 3' NCRs of 117 isolates of South American YFV have been examined, and major deletions and/or duplications of conserved RNA structures have been identified in several wild-type isolates. Nineteen isolates (designated YF-XL isolates) from Brazil, Trinidad, and Venezuela, dating from 1973 to 2001, exhibited a 216-nucleotide (nt) duplication, yielding a tandem repeat of conserved hairpin, stem-loop, dumbbell, and pseudoknot structures. YF-XL isolates were found exclusively within one subclade of South American genotype I YFV. One Brazilian isolate exhibited, in addition to the 216-nt duplication, a deletion of a 40-nt repeated hairpin (RYF) motif (YF-XL-DeltaRYF). To investigate the biological significance of these 3' NCR rearrangements, YF-XL-DeltaRYF and YF-XL isolates, as well as other South American YFV isolates, were evaluated for three phenotypes: growth kinetics in cell culture, neuroinvasiveness in suckling mice, and ability to replicate and produce disseminated infections in Aedes aegypti mosquitoes. YF-XL-DeltaRYF and YF-XL isolates showed growth kinetics and neuroinvasive characteristics comparable to those of typical South American YFV isolates, and mosquito infectivity trials demonstrated that both types of 3' NCR variants were capable of replication and dissemination in a laboratory-adapted colony of A. aegypti.
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页码:3807 / 3821
页数:15
相关论文
共 59 条
[1]   Differential Susceptibility of Aedes aegypti to Infection by the American and Southeast Asian Genotypes of Dengue Type 2 Virus [J].
Armstrong, Philip ;
Rico-Hesse, Rebeca .
VECTOR-BORNE AND ZOONOTIC DISEASES, 2001, 1 (02) :159-+
[2]   RNA recombination between persisting pestivirus and a vaccine strain: Generation of cytopathogenic virus and induction of lethal disease [J].
Becher, P ;
Orlich, M ;
Thiel, HJ .
JOURNAL OF VIROLOGY, 2001, 75 (14) :6256-6264
[3]   A stable full-length yellow fever virus cDNA clone and the role of conserved RNA elements in flavivirus replication [J].
Bredenbeek, PJ ;
Kooi, EA ;
Lindenbach, B ;
Huijkman, N ;
Rice, CM ;
Spaan, WJM .
JOURNAL OF GENERAL VIROLOGY, 2003, 84 :1261-1268
[4]   Comparative phylogenies of yellow fever isolates from Peru and Brazil [J].
Bryant, JE ;
Barrett, ADT .
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2003, 39 (02) :103-118
[5]   Complete genome sequence of Montana Myotis leukoencephalitis virus, phylogenetic analysis and comparative study of the 3′ untranslated region of flaviviruses with no known vector [J].
Charlier, N ;
Leyssen, P ;
Pleij, CWA ;
Lemey, P ;
Billoir, F ;
Van Laethem, K ;
Vandamme, AM ;
De Clercq, E ;
de Lamballerie, X ;
Neyts, J .
JOURNAL OF GENERAL VIROLOGY, 2002, 83 :1875-1885
[6]   American genotype structures decrease dengue virus output from human monocytes and dendritic cells [J].
Cologna, R ;
Rico-Hesse, R .
JOURNAL OF VIROLOGY, 2003, 77 (07) :3929-3938
[7]   Outbreak of jaundice and hemorrhagic fever in the Southeast of Brazil in 2001: Detection and molecular characterization of yellow fever virus [J].
de Filippis, AMB ;
Nogueira, RMR ;
Schatzmayr, HG ;
Tavares, DS ;
Jabor, AV ;
Diniz, SCM ;
Oliveira, JC ;
Moreira, E ;
Miagostovich, MP ;
Costa, EV ;
Galler, R .
JOURNAL OF MEDICAL VIROLOGY, 2002, 68 (04) :620-627
[8]  
Deubel V, 1997, FACTORS IN THE EMERGENCE OF ARBOVIRUS DISEASES, P157
[9]  
Deubel V, 1997, J MED VIROL, V53, P212, DOI 10.1002/(SICI)1096-9071(199711)53:3&lt
[10]  
212::AID-JMV5&gt