Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

被引：52

作者：

Berridge, BR ^{[1
]}

Fuller, JD

de Azavedo, J

Low, DE

Bercovier, H

Frelier, PF

机构：

[1] Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA

[2] Univ Toronto, Dept Microbiol, Toronto, ON, Canada

[3] Mt Sinai Hosp, Toronto, ON M5G 1X5, Canada

[4] Princess Margaret Hosp, Toronto, ON M4X 1K9, Canada

[5] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Clin Microbiol, IL-91010 Jerusalem, Israel

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 09期

关键词：

D O I：

10.1128/JCM.36.9.2778-2781.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

引用

页码：2778 / 2781

页数：4

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