Alternative splicing generates multiple transcripts of the inhibitor of apoptosis protein 1 in Aedes and Culex spp. mosquitoes

被引：6

作者：

Beck, Eric T.

Blair, Carol D.

Black, William C.

Beaty, Barry J. ^{[1
]}

Blitvich, Bradley J.

机构：

[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Arthropod Borne & Infect Dis Lab, Ft Collins, CO 80523 USA

[2] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2007年 / 37卷 / 11期

关键词：

inhibitor of apoptosis; alternative splicing; Aedes mosquitoes; Culex mosquitoes;

D O I：

10.1016/j.ibmb.2007.07.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We determined the sequences of cDNA encoding Inhibitor of Apoptosis Protein 1 (IAP1) homologues from Aedes triseriatus, Aedes albopictus, Aedes aegypti, Culex pipiens and Culex tarsalis. The cDNAs encode translation products that share >= 84% sequence similarity. The IAP1 mRNA of each mosquito species exists as 3-5 distinct variants due to the presence of heterogeneous sequences at the distal end of their 5'UTRs. Partial genomic sequencing upstream of the 5' end of the Ae. triseriatus IAP1 gene, and analysis of the Ae. aegypti genomic sequence, suggest that these mRNA variants are generated by alternative splicing. Each IAP1 mRNA variant from Ae. triseriatus and Cx. pipiens was detected by RT-PCR in all mosquito life-stages and adult tissues examined, and the relative concentration of each Ae. triseriatus IAP mRNA variant in various tissues was determined. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1222 / 1233

页数：12

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