Biodiversity of an Acinetobacter population isolated from activated sludge

被引：27

作者：

Barberio, C ^{[1
]}

Fani, R ^{[1
]}

机构：

[1] Dipartimento Biol Anim & Genet, I-50125 Florence, Italy

来源：

RESEARCH IN MICROBIOLOGY | 1998年 / 149卷 / 09期

关键词：

biodegradation; Acinetobacter; sludge; intraspecific variability; molecular fingerprinting; ARDRA; RAPD;

D O I：

10.1016/S0923-2508(99)80014-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A culturable microbial community from a sewage treatment plant collecting mainly surfactant-enriched wastes was selected on minimal medium containing two nonylphenol ethoxylates as sole carbon source. Biodiversity of the community was assessed on fifty randomly chosen isolates by a combination of molecular techniques. Isolates were first analysed by amplified 16S ribosomal DNA restriction analysis (ARDRA); most of them (75%) were assigned to the genus Acinetobacter on the basis of 16S ribosomal DNA sequencing. Random amplified polymorphic DNA (RAPD) fingerprinting and the analysis of plasmid content showed a high degree of genetic variability and suggested a marked horizontal gene transfer.

引用

页码：665 / 673

页数：9

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