Identification of Staphylococcus aureus and determination of methicillin resistance directly from positive blood cultures by isothermal amplification and a disposable detection device

被引：52

作者：

Goldmeyer, James ^{[3
]}

Li, Haijing ^{[1
]}

Mccormac, Melinda ^{[2
]}

Cook, Steve ^{[4
]}

Stratton, Charles ^{[1
,2
]}

Lemieux, Bertrand ^{[3
]}

Kong, Fluimin ^{[3
]}

Tang, Wen ^{[3
]}

Tang, Yi-Wei ^{[1
,2
]}

机构：

[1] Vanderbilt Univ, Med Ctr, Dept Med, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Med Ctr, Dept Pathol, Nashville, TN 37232 USA

[3] BioHelix Corp, Beverly, MA 01915 USA

[4] Lahey Clin Med Ctr, Burlington, MA 01805 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 04期

关键词：

D O I：

10.1128/JCM.02234-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively.

引用

页码：1534 / 1536

页数：3

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