Pseudo 3D single-walled carbon nanotube film for BSA-free protein chips

被引：12

作者：

Byon, HR

Hong, BJ

Gho, YS

Park, JW

Choi, HC

机构：

[1] Pohang Univ Sci & Technol, Dept Chem, Pohang 790784, South Korea

[2] Pohang Univ Sci & Technol, Dept Life Sci, Pohang, South Korea

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 08期

关键词：

BSA-free system; microarrays; protein chips; nanotubes;

D O I：

10.1002/cbic.200500081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sweet home for proteins. Single-walled carbon nanotube (SWNT) film treated with CDI-Tween20 (CT) has been used as a substrate for a BSA-free protein chip. In the absence of BSA, which is conventionally applied as a nonspecific binding inhibitor, protein A, biotinylated BSA, and even small peptide such as 3 × FLAG immobilized on the CT-functionalized SWNT film substrates recognize their respective counterparts, streptavidin, immunoglobulin G, and antiFLAG. with high specificity. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1331 / 1334

页数：4

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