Binary vectors for sense and antisense expression of arabidopsis ESTs

被引：42

作者：

Mylne, J ^{[1
]}

Botella, JR ^{[1
]}

机构：

[1] Univ Queensland, Dept Bot, Plant Genet Engn Lab, Brisbane, Qld 4072, Australia

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1998年 / 16卷 / 03期

关键词：

antisense constructs; Arabidopsis; binary vector; EST; herbicide resistance; transgenic; sense constructs;

D O I：

10.1023/A:1007520410190

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Our laboratory is interested in devising methods to identify functions for the vast numbers of arabidopsis genes now available. For this purpose, we have constructed a set of binary vectors that will allow the quick production of transgenic arabidopsis plants containing either sense or antisense copies of EST clones obtained from the PRL2 library. These vectors are based on the pSLJ series containing the bialophos resistance (BAR) gene that confers resistance to the herbicide BASTA. Tn addition, our vectors contain a 35S CaMV promoter-polylinker-nos terminator cassette that allows the direct cloning of arabidopsis ESTs in either antisense (pAOV and pAOV2) or sense (pSOV and pSOV2) orientation. We also describe the construction of two additional vectors conferring BASTA resistance and containing the pBluescript polylinker in both orientations inserted between the 35S CaMV promoter and nos terminator (pKMB and pSMB).

引用

页码：257 / 262

页数：6

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