Fluorescence correlation spectroscopy and quantitative cell biology

被引：31

作者：

Levin, MK ^{[1
]}

Carson, JH ^{[1
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Mol Microbiol & Struct Biol, Farmington, CT 06030 USA

来源：

DIFFERENTIATION | 2004年 / 72卷 / 01期

关键词：

fluorescence correlation spectroscopy; avalanche photodiode; photon count histogram; green fluorescent protein;

D O I：

10.1111/j.1432-0436.2004.07201002.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fluorescence correlation spectroscopy (FCS) analyzes fluctuations in fluorescence within a small observation volume. Autocorrelation analysis of FCS fluctuation data can be used to measure concentrations, diffusion properties, and kinetic constants for individual fluorescent molecules. Photon count histogram analysis of fluorescence fluctuation data can be used to study oligomerization of individual fluorescent molecules. If the FCS observation volume is positioned inside a living cell, these parameters can be measured in vivo. FCS can provide the requisite quantitative data for analysis of molecular interaction networks underlying complex cell biological processes.

引用

页码：1 / 10

页数：10

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