Performance of the BACTEC MGIT 960 compared with solid media for detection of Mycobacterium in Bangkok, Thailand

被引:27
作者
Srisuwanvilai, La-ong [2 ]
Monkongdee, Patama [3 ]
Podewils, Laura Jean [1 ]
Ngamlert, Keerataya [2 ]
Pobkeeree, Vallerut [3 ]
Puripokai, Panitchaya [2 ]
Kanjanamongkolsiri, Photjanart [2 ]
Subhachaturas, Wonchat [2 ]
Akarasewi, Pasakorn [3 ]
Wells, Charles D. [1 ]
Tappero, Jordan W. [1 ,3 ]
Varma, Jay K. [1 ,3 ]
机构
[1] US Ctr Dis Control & Prevent, Div TB Eliminat, Atlanta, GA 30333 USA
[2] Bangkok Metropolitan Hlth Adm, Dept Hlth, Hlth Lab Div, Bangkok 10330, Thailand
[3] US CDC Collaborat, Thailand Minist Publ Hlth, Bangkok 11000, Thailand
关键词
tuberculosis; diagnosis; MGIT; surveillance; HIV/AIDS;
D O I
10.1016/j.diagmicrobio.2008.02.015
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Controlled trials have demonstrated that liquid media culture (LMC) is superior to solid media culture for diagnosis of Mycobacterium tuberculosis (MTB), but there is limited evidence about its performance in resource-limited settings. We evaluated the performance of LMC in a demonstration project in Bangkok, Thailand. Sputum specimens from persons with suspected or clinically diagnosed tuberculosis were inoculated in parallel on solid (Lowenstein-Jensen [LJ]) and liquid (mycobacterial growth indicator tube [MGIT 960]) media. Biochemical tests identified isolates as MTB or nontuberculosis mycobacteria (NTM). Of 2566 specimens received from October 2004 to September 2006, 1355 (53%) were culture positive by MGIT compared with 10 13 (39%) by LJ. Median time to growth for MGIT was significantly less than LJ: I I versus 27 days. Of 1417 isolates detected by at least 1 media, 1255 (86%) were identified as MTB and 162 (11%) NTM. MGIT improved speed and sensitivity of MTB isolation and drug susceptibility testing, regardless of HIV status. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:402 / 407
页数:6
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