Alternate polyadenylation in human mRNAs: A large-scale analysis by EST clustering

被引：106

作者：

Gautheret, D ^{[1
]}

Poirot, O ^{[1
]}

Lopez, F ^{[1
]}

Audic, S ^{[1
]}

Claverie, JM ^{[1
]}

机构：

[1] CNRS, F-13402 Marseille 20, France

来源：

GENOME RESEARCH | 1998年 / 8卷 / 05期

关键词：

D O I：

10.1101/gr.8.5.524

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alternate polyadenylation is all important post-transcriptional regulatory process now open to large-scale analysis by use of cDNA databases. We clustered 164,000 expressed sequence tags (ESTs) into similar to 15,000 groups and aligned each group to a putative mRNA 3' end. By use of stringent criteria to discard artifactual mRNA extremities, clear evidence for alternate polyadenylation was obtained in 189 of the 1000 EST clusters studied. A number of previously unreported polyadenylation sites were identified, together with possible instances of tissue-specific differential polyadenylation. This study demonstrates that, besides quantitative aspects of gene expression, the distribution of alternate mRNA forms can be analyzed through EST sampling.

引用

页码：524 / 530

页数：7

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