A comparison of MS2-based label-free quantitative proteomic techniques with regards to accuracy and precision

被引：17

作者：

Colaert, Niklaas ^{[1
,2
]}

Vandekerckhove, Joel ^{[1
,2
]}

Gevaert, Kris ^{[1
,2
]}

Martens, Lennart ^{[1
,2
]}

机构：

[1] VIB, Dept Med Prot Res, Ghent, Belgium

[2] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium

来源：

PROTEOMICS | 2011年 / 11卷 / 06期

关键词：

Accuracy; Bioinformatics; Label free; Precision; Quality control; Quantitative proteomics; QUANTIFICATION; PROTEIN;

D O I：

10.1002/pmic.201000521

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The advent of algorithms for fragmentation spectrum-based label-free quantitative proteomics has enabled straightforward quantification of shotgun proteomic experiments. Despite the popularity of these approaches, few studies have been performed to assess their performance. We have therefore profiled the precision and the accuracy of three distinct relative label-free methods on both the protein and the proteome level. We derived our test data from two well-characterized publicly available quantitative data sets.

引用

页码：1110 / 1113

页数：4

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