miR-137 Is Frequently Down-Regulated in Gastric Cancer and Is a Negative Regulator of Cdc42

被引：123

作者：

Chen, Qingjiang ^{[1
]}

Chen, Xiaobing ^{[2
]}

Zhang, Mingzhi ^{[1
]}

Fan, Qingxia ^{[1
]}

Luo, Suxia ^{[2
]}

Cao, Xinguang ^{[2
]}

机构：

[1] Zhengzhou Univ, Dept Med Oncol, Affiliated Hosp 1, Zhengzhou 450000, Peoples R China

[2] Zhengzhou Univ, Dept Internal Med Oncol, Henan Canc Hosp, Affiliated Canc Hosp, Zhengzhou 450000, Peoples R China

来源：

DIGESTIVE DISEASES AND SCIENCES | 2011年 / 56卷 / 07期

关键词：

miR-137; Gastric cancer; Hypermethylation; Cdc42; MICRORNAS; DIFFERENTIATION; PROLIFERATION; GTPASES; DEATH; RAC1;

D O I：

10.1007/s10620-010-1536-3

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

100201 [内科学];

摘要：

MicroRNAs (miRNAs) are a class of small (19-25 nucleotides) noncoding RNAs that regulate the expressions of a wide variety of genes, including some involved in cancer development. Some recent studies show that DNA methylation contributes to down-regulation of microRNA-137 (miR-137) during tumorigenesis. Whether down-regulation of miR-137 also exists in gastric cancer is unknown. Our aim was to test the hypothesis that down-regulation of miR-137 also exists in gastric cancer. Expression of levels of miR-137 were examined using real-time PCR on paired gastric cancer and adjacent non-cancerous tissues. The methylation status is detected by MSP. Results show that miR-137 is downregulated by hypermethylation of the promoter in gastric cancer tissues. Epigenetic silencing of miR-137 induced an up-regulation of its targets, Cdc42. Restoration of the miR-137 expression in gastric cancer cell lines downregulated the Cdc42 expression. Restoration of the miR-137 and inactivation of Cdc42 induce apoptosis and cell cycle G1 arrest in gastric cancer cells. Furthermore, the miR-137 expression was found to be inversely correlated with CDC42 expression in gastric caner. miR-137 is frequently down-regulated in gastric cancer and is a negative regulator of Cdc42.

引用

页码：2009 / 2016

页数：8

共 17 条

[1]

Epigenetic Silencing of miR-137 Is an Early Event in Colorectal Carcinogenesis [J].