Preparation and properties of amperometric uric acid sensors

被引:38
作者
Kuwabata, S [1 ]
Nakaminami, T [1 ]
Ito, S [1 ]
Yoneyama, H [1 ]
机构
[1] Osaka Univ, Fac Engn, Dept Appl Chem, Osaka 5650871, Japan
关键词
uric acid sensor; uricase; electron mediator;
D O I
10.1016/S0925-4005(98)00258-5
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Uricase molecules (UOx) were immobilized to a self-assembled monolayer of 2-aminoethanethiolate prepared on an Au electrode substrate by casting phosphate buffer solution containing UOx and glutaraldehyde as a cross-linking agent, followed by drying. The prepared electrode (UOx/Au) exhibited amperometric responses to uric acid with use of [Fe(CN)(6)](3-) dissolved in solution as an electron mediator at rates much higher than those obtained with use of dissolved UOx. The sensitivity of the UOx/Au electrode was influenced by not only the amount of glutaraldehyde in the casting solution but also the drying time and the temperature after casting the solution. The electrodes prepared under the best preparation conditions showed amperometric responses to uric acid up to 0.6 mmol dm(-3), which covers the normal concentration of uric acid in the human blood. (C) 1998 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:72 / 77
页数:6
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