Determination of ionisation constants of organic bases in aqueous methanol solutions using capillary electrophoresis

被引：42

作者：

Buckenmaier, SMC

McCalley, DV ^{[1
]}

Euerby, MR

机构：

[1] Univ W England, Ctr Res Analyt Methods & Sensors, Bristol BS16 1QY, Avon, England

[2] AstraZeneca R&D Charnwood Lunc, Pharmaceut & Analyt R&D, Loughborough LE11 5RH, Leics, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1026卷 / 1-2期

关键词：

dissociation constants; triptylines; diphenhydramine; quinine; codeine; procainamide; benzylamine;

D O I：

10.1016/j.chroma.2003.11.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The pK(a) of eight organic bases was determined in aqueous and aqueous methanol solutions of 0-70% (v/v) methanol using capillary electrophoresis. The bases investigated include compounds commonly used to test the activity of RP columns in HPLC. The variation of pK(a) with temperature in aqueous methanol solutions was also investigated and found to closely resemble temperature coefficients reported for bases in purely aqueous solutions. pK(a) values determined by CE were compared to those reported using NMR spectroscopy. The good agreement of the results is evidence that either technique is suitable to perform pK(a) measurements. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：251 / 259

页数：9

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