Crystal growth and solubility studies of Aspergillus niger acid proteinase A in the presence of both a salt and an organic solvent

被引：3

作者：

Soga, T

Sasaki, H

Tanokura, M ^{[1
]}

Ataka, M

机构：

[1] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan

[2] Natl Inst Biosci & Human Technol, Higashi Ku, Tsukuba, Ibaraki 3058566, Japan

来源：

JOURNAL OF CRYSTAL GROWTH | 1999年 / 196卷 / 2-4期

关键词：

solubility; proteinase A; Aspergillus niger; dimethylsulfoxide; ammonium sulfate;

D O I：

10.1016/S0022-0248(98)00825-2

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The quality of proteinase A crystals improves considerably, as judged from the X-ray diffraction limit, in the presence of an organic solvent, dimethylsulfoxide (DMSO). The effects of DMSO have therefore been studied through the observation of crystallization and protein concentration versus time. It was found that DMSO increases the nucleation rate, and decreases the crystal solubility as does ammonium sulfate. It is suggested that organic solvents may be used to alter the protein crystal growth usually obtained by salting-out. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：291 / 296

页数：6

共 6 条

[1] Phase diagram of crystallization of Aspergillus niger acid proteinase A, a non-pepsin-type acid proteinase [J].