Respiratory and systemic humoral and cellular immune responses of pigs to a heterosubtypic influenza A virus infection

被引:90
作者
Heinen, PP [1 ]
de Boer-Luijtze, EA [1 ]
Bianchi, ATJ [1 ]
机构
[1] Inst Anim Sci & Hlth ID Lelystad BV, Dept Mammalian Virol, NL-8200 AB Lelystad, Netherlands
关键词
D O I
10.1099/0022-1317-82-11-2697
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The level of heterosubtypic immunity (Het-I) and the immune mechanisms stimulated by a heterosubtypic influenza virus infection were investigated in pigs. Pigs are natural hosts for influenza virus and, like humans, they host both subtypes H1N1 and H3N2. Marked Het-I was observed when pigs were infected with H1N1 and subsequently challenged with H3N2. After challenge with H3N2, pigs infected earlier with H1N1 did not develop fever and showed reduced virus excretion compared with non-immune control pigs. In addition, virus transmission to unchallenged group-mates could be shown by virus isolation in the non-immune control group but not in the group infected previously with H1N1. Pigs infected previously with homologous H3N2 virus were protected completely. After challenge with H3N2, pigs infected previously with H1N1 showed a considerable increase in serum IgG titre to the conserved extracellular domain of M2 but not to the conserved nucleoprotein. These results suggest that antibodies against external conserved epitopes can have an important role in broad-spectrum immunity. After primary infection with both H1N1 and H3N2, a long-lived increase was observed in the percentage of CD8(+) T cells in the lungs and in the lymphoproliferation response in the blood. Upon challenge with H3N2, pigs infected previously with H1N1 again showed an increase in the percentage of CD8(+) T cells in the lungs, whereas pigs infected previously with H3N2 did not, suggesting that CD84(+) T cells also have a role in Het-I. To confer broad-spectrum immunity, future vaccines should induce antibodies and CD8(+)T cells against conserved antigens.
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页码:2697 / 2707
页数:11
相关论文
共 46 条
  • [1] RECENT H3N2 SWINE INFLUENZA-VIRUS WITH HEMAGGLUTININ AND NUCLEOPROTEIN GENES SIMILAR TO 1975 HUMAN STRAINS
    BIKOUR, MH
    FROST, EH
    DESLANDES, S
    TALBOT, B
    WEBER, JM
    ELAZHARY, Y
    [J]. JOURNAL OF GENERAL VIROLOGY, 1995, 76 : 697 - 703
  • [2] ANTIBODY-RESPONSE TO THE M2-PROTEIN OF INFLUENZA A-VIRUS EXPRESSED IN INSECT CELLS
    BLACK, RA
    ROTA, PA
    GORODKOVA, N
    KLENK, HD
    KENDAL, AP
    [J]. JOURNAL OF GENERAL VIROLOGY, 1993, 74 : 143 - 146
  • [3] Antigenic and genetic analyses of H1N1 influenza A viruses from European pigs
    Brown, IH
    Ludwig, S
    Olsen, CW
    Hannoun, C
    Scholtissek, C
    Hinshaw, VS
    Harris, PA
    McCauley, JW
    Strong, I
    Alexander, DJ
    [J]. JOURNAL OF GENERAL VIROLOGY, 1997, 78 : 553 - 562
  • [4] Multiple genetic reassortment of avian and human influenza A viruses in European pigs, resulting in the emergence of an H1N2 virus of novel genotype
    Brown, IH
    Harris, PA
    McCauley, JW
    Alexander, DJ
    [J]. JOURNAL OF GENERAL VIROLOGY, 1998, 79 : 2947 - 2955
  • [5] ANTIGENIC AND SEQUENCE-ANALYSIS OF H3 INFLUENZA-VIRUS HEMAGGLUTININS FROM PIGS IN ITALY
    CASTRUCCI, MR
    CAMPITELLI, L
    RUGGIERI, A
    BARIGAZZI, G
    SIDOLI, L
    DANIELS, R
    OXFORD, JS
    DONATELLI, I
    [J]. JOURNAL OF GENERAL VIROLOGY, 1994, 75 : 371 - 379
  • [6] GENETIC REASSORTMENT BETWEEN AVIAN AND HUMAN INFLUENZA-A VIRUSES IN ITALIAN PIGS
    CASTRUCCI, MR
    DONATELLI, I
    SIDOLI, L
    BARIGAZZI, G
    KAWAOKA, Y
    WEBSTER, RG
    [J]. VIROLOGY, 1993, 193 (01) : 503 - 506
  • [7] De Bruin TGM, 2000, VIRAL IMMUNOL, V13, P511
  • [8] Antigenic drift in swine influenza H3 haemagglutinins with implications for vaccination policy
    de Jong, JC
    van Nieuwstadt, AP
    Kimman, TG
    Loeffen, WLA
    Bestebroer, TM
    Bijlsma, K
    Osterhaus, ADME
    Claas, ECJ
    [J]. VACCINE, 1999, 17 (11-12) : 1321 - 1328
  • [9] Accessing complexity: The dynamics of virus-specific T cell responses
    Doherty, PC
    Christensen, JP
    [J]. ANNUAL REVIEW OF IMMUNOLOGY, 2000, 18 : 561 - 592
  • [10] RECOMBINANT VACCINIA VIRUSES AND THE DEVELOPMENT OF IMMUNIZATION STRATEGIES USING INFLUENZA-VIRUS
    DOHERTY, PC
    ALLAN, W
    BOYLE, DB
    COUPAR, BEH
    ANDREW, ME
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1989, 159 (06) : 1119 - 1122