GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells

被引：25

作者：

Buza-Vidas, Natalija ^{[1
,2
]}

Duarte, Sara ^{[1
]}

Luc, Sidinh ^{[1
]}

Bouriez-Jones, Tiphaine ^{[1
]}

Woll, Petter S. ^{[1
]}

Jacobsen, Sten Eirik Waelgaard ^{[1
,3
]}

机构：

[1] Univ Oxford, Haematopoiet Stem Cell Lab, Weatherall Inst Mol Med, John Radcliffe Hosp, Oxford OX3 9DS, England

[2] Univ Edinburgh, Inst Stem Cell Res, Edinburgh, Midlothian, Scotland

[3] Univ Oxford, MRC, Weatherall Inst Mol Med, John Radcliffe Hosp,Mol Haematol Unit, Oxford OX3 9DS, England

来源：

BLOOD | 2011年 / 118卷 / 05期

基金：

英国医学研究理事会; 欧盟第七框架计划;

关键词：

TRANSCRIPTION FACTOR GATA-3; LINEAGE COMMITMENT; GENE-EXPRESSION; IN-VIVO; DIFFERENTIATION; PROGENITORS; ONTOGENY; DELETION; RESCUE; LOCUS;

D O I：

10.1182/blood-2011-02-338046

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

GATA3 has been identified as a master regulator of T helper cells, as well as being important for early thymic progenitors and T-cell commitment. However, Gata3 expression initiates already at the hematopoietic stem cell (HSC) level, implicating a potential role also in the regulation of HSCs. Herein we used a conditional Gata3 knockout strategy in which Gata3 expression was completely deleted from the earliest stage of embryonic hematopoietic development after emergence of HSCs from hemogenic endothelium. Through a detailed analysis of HSCs at the phenotypic and functional level, we demonstrate that steady-state levels of HSCs are normal in Gata3(fl/fl)Vav-Cre(tg/+) mice. Moreover, through long-term primary and secondary transplantation experiments, we also unequivocally demonstrate that Gata3 has a redundant role in post-transplantation HSC self-renewal. (Blood. 2011; 118(5):1291-1293)

引用

页码：1291 / 1293

页数：3

共 24 条

[1]

Identification of Flt3+ lympho-myeloid stem cells lacking erythro-megakaryocytic potential:: A revised road map for adult blood lineage commitment [J].