Interfamily Transfer of Tomato Ve1 Mediates Verticillium Resistance in Arabidopsis

被引:227
作者
Fradin, Emilie F. [1 ,2 ]
Abd-El-Haliem, Ahmed [1 ]
Masini, Laura [1 ]
van den Berg, Grardy C. M. [1 ]
Joosten, Matthieu H. A. J. [1 ,2 ]
Thomma, Bart P. H. J. [1 ,2 ]
机构
[1] Wageningen Univ, Phytopathol Lab, NL-6708 PB Wageningen, Netherlands
[2] Ctr BioSyst Genom, NL-6700 AB Wageningen, Netherlands
关键词
RECEPTOR-LIKE PROTEINS; LEUCINE-RICH REPEAT; DISEASE RESISTANCE; CLADOSPORIUM-FULVUM; CELL-DEATH; RECOGNITIONAL SPECIFICITY; INNATE IMMUNITY; PLANT-PATHOGEN; GENE FAMILY; KINASE;
D O I
10.1104/pp.111.180067
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Vascular wilts caused by soil-borne fungal species of the Verticillium genus are devastating plant diseases. The most common species, Verticillium dahliae and Verticillium albo-atrum, have broad host ranges and are notoriously difficult to control. Therefore, genetic resistance is the preferred method for disease control. Only from tomato (Solanum lycopersicum) has a Verticillium resistance locus been cloned, comprising the Ve1 gene that encodes a receptor-like protein-type cell surface receptor. Due to lack of a suitable model for receptor-like protein (RLP)-mediated resistance signaling in Arabidopsis (Arabidopsis thaliana), so far relatively little is known about RLP signaling in pathogen resistance. Here, we show that Ve1 remains fully functional after interfamily transfer to Arabidopsis and that Ve1-transgenic Arabidopsis is resistant to race 1 but not to race 2 strains of V. dahliae and V. albo-atrum, nor to the Brassicaceae-specific pathogen Verticillium longisporum. Furthermore, we show that signaling components utilized by Ve1 in Arabidopsis to establish Verticillium resistance overlap with those required in tomato and include SERK3/BAK1, EDS1, and NDR1, which strongly suggests that critical components for resistance signaling are conserved. We subsequently investigated the requirement of SERK family members for Ve1 resistance in Arabidopsis, revealing that SERK1 is required in addition to SERK3/BAK1. Using virus-induced gene silencing, the requirement of SERK1 for Ve1-mediated resistance was confirmed in tomato. Moreover, we show the requirement of SERK1 for resistance against the foliar fungal pathogen Cladosporium fulvum mediated by the RLP Cf-4. Our results demonstrate that Arabidopsis can be used as model to unravel the genetics of Ve1-mediated resistance.
引用
收藏
页码:2255 / 2265
页数:11
相关论文
共 80 条
[1]   Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis [J].
Aarts, N ;
Metz, M ;
Holub, E ;
Staskawicz, BJ ;
Daniels, MJ ;
Parker, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) :10306-10311
[2]   The Arabidopsis thaliana SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASES1 and 2 control male sporogenesis [J].
Albrecht, C ;
Russinova, E ;
Hecht, V ;
Baaijens, E ;
de Vries, S .
PLANT CELL, 2005, 17 (12) :3337-3349
[3]   Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE proteins serve brassinosteroid-dependent and -independent signaling pathways [J].
Albrecht, Catherine ;
Russinova, Eugenia ;
Kemmerling, Birgit ;
Kwaaitaal, Mark ;
de Vries, Sacco C. .
PLANT PHYSIOLOGY, 2008, 148 (01) :611-619
[4]  
[Anonymous], CURR OPIN PLANT BIOL
[5]  
[Anonymous], PLANT J
[6]  
Barbara DJ, 2003, MOL PLANT PATHOL, V4, P297, DOI [10.1046/j.1364-3703.2003.00172.x, 10.1046/J.1364-3703.2003.00172.X]
[7]   The HcrVf2 gene from a wild apple confers scab resistance to a transgenic cultivated variety [J].
Belfanti, E ;
Silfverberg-Dilworth, E ;
Tartarini, S ;
Patocchi, A ;
Barbieri, M ;
Zhu, J ;
Vinatzer, BA ;
Gianfranceschi, L ;
Gessler, C ;
Sansavini, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (03) :886-890
[8]   Mapping of verticillium wilt resistance genes in cotton [J].
Bolek, Y ;
El-Zik, KM ;
Pepper, AE ;
Bell, AA ;
Magill, CW ;
Thaxton, PM ;
Reddy, OUK .
PLANT SCIENCE, 2005, 168 (06) :1581-1590
[9]   NDR1, A LOCUS OF ARABIDOPSIS-THALIANA THAT IS REQUIRED FOR DISEASE RESISTANCE TO BOTH A BACTERIAL AND A FUNGAL PATHOGEN [J].
CENTURY, KS ;
HOLUB, EB ;
STASKAWICZ, BJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (14) :6597-6601
[10]   Molecular cloning of a potential Verticillium dahliae resistance gene SlVe1 with multi-site polyadenylation from Solanum licopersicoides [J].
Chai, YR ;
Zhao, LX ;
Liao, ZH ;
Sun, XF ;
Zuo, KJ ;
Zhang, L ;
Wang, SG ;
Tang, KX .
DNA SEQUENCE, 2003, 14 (05) :375-384