Micelles by self-assembling peptide-conjugate amphiphile: synthesis and structural characterization

被引：11

作者：

Accardo, Antonella ^{[1
,2
]}

Tesauro, Diego ^{[1
,2
]}

Del Pozzo, Luigi ^{[1
,2
]}

Mangiapia, Gaetano ^{[3
]}

Paduano, Luigi ^{[3
]}

Morelli, Giancarlo ^{[1
,2
]}

机构：

[1] Univ Naples Federico 2, CIRPeB, Dept Biol Sci, I-80134 Naples, Italy

[2] IBB CNR, I-80134 Naples, Italy

[3] Univ Naples Federico 2, Dept Chem, I-80126 Naples, Italy

来源：

JOURNAL OF PEPTIDE SCIENCE | 2008年 / 14卷 / 08期

关键词：

peptide-conjugate amphiphile (PCA); CCK8; peptide; micelles small-angle neutron scattering;

D O I：

10.1002/psc.1024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemical synthesis by solid-phase methods of a novel amphiphilic peptide, peptide-conjugate amphiphile (PCA), containing in the same molecule three different functions: (i) the N.N-bis[2-[bis(carboxy-ethyl)arnino]ethyl]-L-glutamic acid (DTPAGlu) chelating agent. (ii) the CCK8 bioactive peptide, and (iii) a hydrophobic moiety containing four alkyl chains with 18 carbon atoms each, is reported. In water solution at. pH 7.4, PCA self-assembles in very stable micelles at very low concentration [critical micellar concentration (cmc) values of 5 x 10(-7) mol kg(-1)] as confirmed by fluorescence spectroscopy. The structural characterization, obtained with small-angle neutron scattering (SANS) measurements. indicates that the aggregates are substantially represented by ellipsoidal micelles with an aggregation number of 39 +/- 2 and the two micellar axes of about: 52 and 26 A. Copyright (C) 2008 European Peptide Society and John Wiley & Sons, Ltd.

引用

页码：903 / 910

页数：8

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