Effect of insulin nitration by peroxynitrite on its biological activity

被引：30

作者：

Chi, Q ^{[1
]}

Wang, TL ^{[1
]}

Huang, KX ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Chem, Wuhan 430074, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 330卷 / 03期

基金：

中国国家自然科学基金;

关键词：

peroxynitrite; insulin; tyrosine nitration; mass spectrometry;

D O I：

10.1016/j.bbrc.2005.03.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin may be a potential target of ONOO- during conditions involving accelerated rates of oxygen radical and nitric oxide generation in pancreatic islet beta-cells. To evaluate the effect of protein tyrosine nitration on insulin function, the porcine insulin was nitrated by ONOO-. Insulin nitration was confirmed by spectral changes, Native-PAGE, and mass spectrometry. The bioassay results show that mono-nitro-insulin affected its receptor binding and hypoglycemic capacities to a certain extent, but still hold high biological activities. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：791 / 796

页数：6

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