Repair of osteochondral defects with hyaluronan- and polyester-based scaffolds

被引:150
作者
Solchaga, LA
Temenoff, JS
Gao, JZ
Mikos, AG
Caplan, AI
Goldberg, VM
机构
[1] Case Western Reserve Univ, Millis Sci Ctr, Dept Orthopaed, Sch Med, Cleveland, OH 44106 USA
[2] Univ Hosp Cleveland, Cleveland, OH 44106 USA
[3] Case Western Reserve Univ, Skeletal Res Ctr, Dept Biol, Cleveland, OH 44106 USA
[4] Rice Univ, Dept Bioengn, Houston, TX 77251 USA
关键词
tissue engineering; cartilage repair; scaffolds; hyaluronan; PLLA; PLGA;
D O I
10.1016/j.joca.2004.12.016
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Objective: The natural repair of osteochondral defects can be enhanced with biocompatible, biodegradable materials that support the repair process. It is our hypothesis that hyaluronan-based scaffolds are superior to synthetic scaffolds because they provide biological cues. We tested this thesis by comparing two hyaluronan-based scaffolds [auto cross-linked polysaccharide polymer (ACP (TM)) and HYAFF (R)-11] to polyester-based scaffolds [poly(DL-lactic-co-glycolic acid) (PLGA) and poly(L-lactic acid) (PLLA)] with similar pore size, porosity and degradation times. Design: Fifty-four rabbits received bilateral osteochondral defects. One defect received a hyaluronan-based scaffold and the contralateral defect received the corresponding polyester-based scaffold. Rabbits were euthanized 4, 12 and 20 weeks after surgery and the condyles dissected and processed for histology. Results: Only ACP (TM)-treated defects presented bone at the base of the defect at 4 weeks. At 12 weeks, only defects treated with rapidly dissolving implants (ACP (TM) and PLGA) presented bone reconstitution consistently, while bone was present in only one third of those treated with slowly dissolving scaffolds (HYAFF (R)-11 and PLLA). After 20 weeks, the articular surface of PLGA-treated defects presented fibrillation more frequently than in ACP (TM)-treated defects. The surface of defects treated with slowly dissolving scaffolds presented more cracks and fissures. Conclusions: The degradation rate of the scaffolds is critical for the repair process. Slowly dissolving scaffolds sustain thicker cartilage at the surface but, it frequently presents cracks and discontinuities. These scaffolds also delay bone formation at the base of the defects. Hyaluronan-based scaffolds appear to allow faster cell infiltration leading to faster tissue formation. The degradation of ACP (TM) leads to rapid bone formation while the slow degradation of HYAFF (R)-11 prolongs the presence of cartilage and delays endochondral bone formation. (c) 2005 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:297 / 309
页数:13
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