Multi-residue method for non-steroidal anti-inflammatory drugs in plasma using high-performance liquid chromatography photodiode-array detection -: Method description and comprehensive in-house validation

A multi-residue high-performance liquid chromatography (HPLC) method with photodiode-array detection is presented for the determination of 12 non-steroidal anti-inflammatory drugs (NSAIDs) in plasma. This method has been validated under the consideration of actual alpha- and beta-errors according to an in-house validation concept based on a fractional factorial experiment. A wide range of matrices and other influencing factors have been included in the validation experiment. In order to assess the method's performance the power curve, which demonstrates the detection power of the analytical method, was computed and CCalpha and CCbeta values were calculated. (C) 1998 Elsevier Science BN. All rights reserved.