Mapping protein-protein interactions with combinatorial biology methods

被引：20

作者：

Pelletier, J

Sidhu, S

机构：

[1] Univ Montreal, Dept Chim, Montreal, PQ H3C 3J7, Canada

[2] Genentech Inc, Dept Prot Engn, San Francisco, CA 94080 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(00)00225-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extremely diverse, DNA-encoded libraries of peptides and proteins have been constructed that include a linkage between each polypeptide and the encoding DNA. Library members can be selected by virtue of a particular binding specificity, and their protein sequence can be deduced from the sequence of the cognate DNA. Such combinatorial biology methods have proven invaluable in both identifying natural protein-protein interactions and also in mapping the specificities and energetics of these interactions in fine detail.

引用

收藏

页码：340 / 347

页数：8

相关论文

共 69 条

[1] A heterodimeric coiled-coil peptide pair selected in vivo from a designed library-versus-library ensemble [J].

Arndt, KM ;

Pelletier, JN ;

Müller, KM ;

Alber, T ;

Michnick, SW ;

Plückthun, A .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 295 (03) :627-639

[2] Protein recruitment systems for the analysis of protein±protein interactions [J].

Aronheim, A .

METHODS, 2001, 24 (01) :29-34

[3] Protein recruitment systems for the analysis of protein-protein interactions [J].

Aronheim, A .

BIOCHEMICAL PHARMACOLOGY, 2000, 60 (08) :1009-1013

[4] Epidermal growth factor receptor dimerization monitored in live cells [J].

Blakely, BT ;

Rossi, FMV ;

Tillotson, B ;

Palmer, M ;

Estelles, A ;

Blau, HM .

NATURE BIOTECHNOLOGY, 2000, 18 (02) :218-222

[5] Oligomerization of G-protein-coupled transmitter receptors [J].

Bouvier, M .

NATURE REVIEWS NEUROSCIENCE, 2001, 2 (04) :274-286

[6] Identification of natural ligands for SH2 domains from a phage display cDNA library [J].

Cochrane, D ;

Webster, C ;

Masih, G ;

McCafferty, J .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 297 (01) :89-97

[7] SCREENING FOR RECEPTOR LIGANDS USING LARGE LIBRARIES OF PEPTIDES LINKED TO THE C-TERMINUS OF THE LAC REPRESSOR [J].

CULL, MG ;

MILLER, JF ;

SCHATZ, PJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1865-1869

[8] Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex [J].

Damelin, M ;

Silver, PA .

MOLECULAR CELL, 2000, 5 (01) :133-140

[9] Why FRET over genomics? [J].

De Angelis, DA .

PHYSIOLOGICAL GENOMICS, 1999, 1 (02) :93-99

[10] Modified phage peptide libraries as a tool to study specificity of phosphorylation and recognition of tyrosine containing peptides [J].

Dente, L ;

Vetriani, C ;

Zucconi, A ;

Pelicci, G ;

Lanfrancone, L ;

Pelicci, PG ;

Cesareni, G .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 269 (05) :694-703

← 1 2 3 4 5 6 7 →