Identification and characterization of metallothionein III (growth inhibitory factor) from porcine brain

被引：18

作者：

Chen, CF ^{[1
]}

Wang, SH ^{[1
]}

Lin, LY ^{[1
]}

机构：

[1] NATL TSING HUA UNIV, INST RADIAT BIOL, HSINCHU, TAIWAN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1996年 / 115卷 / 01期

关键词：

brain; cDNA sequence; characterization; gene expression; growth inhibitory factor; isolation; metallothionein III; porcine;

D O I：

10.1016/0305-0491(96)00080-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porcine metallothionein III (MTIII) was isolated from brain tissue by the combination of gel filtration and anion-exchange chromatographies. The identity of MTIII was confirmed by comparing the chromatographic characteristics to other MT isoforms isolated from porcine liver. Porcine MTIII has a low molecular weight and a pl of 4.1. This protein contains both zinc and copper, and the zinc can be replaced by cadmium. Using reverse transcriptase-polymerase chain reaction, a 0.2 kb DNA fragment can be amplified from the porcine brain mRNA. This DNA fragment was demonstrated to contain MTIII coding region after cloning and sequencing. The revealed DNA sequence can be translated into 68 amino acids and shows a common structural characteristic of MTIII from other species. Northern blot analysis indicated chat MTIII mRNA is expressed in every specified region of the porcine brain examined here.

引用

页码：27 / 32

页数：6

共 20 条

[1]

Ausubel FA, 1995, CURRENT PROTOCOLS MO

[2] CONSTITUTIVE EXPRESSION OF METALLOTHIONEIN GENES IN MOUSE-BRAIN [J].