Pseudomonas aeruginosa LasA protease and corneal infections

被引:14
作者
Alionte, LG
Cannon, BM
White, CD
Caballero, AR
O'Callaghan, RJ
Hobden, JA
机构
[1] Wayne State Univ, Sch Med, Dept Immunol & Microbiol, Detroit, MI 48201 USA
[2] LSU, Sch Med, Dept Microbiol Immunol & Parasitol, New Orleans, LA USA
关键词
corneal ulceration; keratitis; Pseudomonas keratitis; proteinases;
D O I
10.1076/ceyr.22.4.266.5509
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose. A mutant strain of Pseudomonas aeruginosa deficient in LasA protease (staphylolytic protease) has been described as having reduced ocular virulence, suggesting that LasA is a major virulence factor. This study was undertaken to provide further genetic analysis of the role of P. aeruginosa LasA protease in ocular infections. Methods. LasA protease-deficient mutants of P. aeruginosa PAO1-V and ATCC 19660 were constructed by allelic replacement. Mutants and their respective wild type parent strains were evaluated for virulence and growth in the eye using mouse scarification and rabbit intrastromal injection models of keratitis. Results. LasA protease-deficient mutants of both strains were as virulent as wild type strains, growing to 4 to 6 log(10) CFU/cornea and causing significant ocular pathology in the mouse (P > 0.42) and rabbit (P > 0.53). Conclusions. These data show that LasA protease is not a major corneal virulence factor, suggesting that the main mechanism of corneal damage has yet to be definitively identified.
引用
收藏
页码:266 / 271
页数:6
相关论文
共 35 条
[1]   Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa [J].
Braun, P ;
de Groot, A ;
Bitter, W ;
Tommassen, J .
JOURNAL OF BACTERIOLOGY, 1998, 180 (13) :3467-3469
[2]  
Crosa Jorge H., 1994, P365
[3]  
De Bruijn Frans J., 1994, P387
[4]   Protease IV, a unique extracellular protease and virulence factor from Pseudomonas aeruginosa [J].
Engel, LS ;
Hill, JM ;
Caballero, AR ;
Green, LC ;
O'Callaghan, RJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (27) :16792-16797
[5]  
Engel LS, 1997, INVEST OPHTH VIS SCI, V38, P1535
[6]  
Engel LS, 1998, INVEST OPHTH VIS SCI, V39, P662
[7]   CLONING AND TRANSCRIPTIONAL REGULATION OF THE ELASTASE LASA GENE IN MUCOID AND NONMUCOID PSEUDOMONAS-AERUGINOSA [J].
GOLDBERG, JB ;
OHMAN, DE .
JOURNAL OF BACTERIOLOGY, 1987, 169 (03) :1349-1351
[8]   Bacterial proteases and adherence of Pseudomonas aeruginosa to mouse cornea [J].
Gupta, SK ;
Masinick, SA ;
Hobden, JA ;
Berk, RS ;
Hazlett, LD .
EXPERIMENTAL EYE RESEARCH, 1996, 62 (06) :641-649
[9]   A substitution at His-120 in the LasA protease of Pseudomonas aeruginosa blocks enzymatic activity without affecting propeptide processing or extracellular secretion [J].
Gustin, JK ;
Kessler, E ;
Ohman, DE .
JOURNAL OF BACTERIOLOGY, 1996, 178 (22) :6608-6617
[10]  
GUSTIN JK, 1998, THESIS U TENNESSEE M