Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes

被引:180
作者
Dhonukshe, Pankaj [1 ,7 ]
Grigoriev, Ilya [2 ]
Fischer, Rainer [3 ]
Tominaga, Motoki [4 ]
Robinson, David G. [5 ]
Hasek, Jiri [6 ]
Paciorek, Tomasz [1 ]
Petrasek, Jan [8 ]
Seifertova, Daniela [8 ]
Tejos, Ricardo [12 ,13 ,14 ]
Meisel, Lee A. [14 ]
Zazimalova, Eva [8 ]
Gadella, Theodorus W. J., Jr. [7 ]
Stierhof, York-Dieter [1 ]
Ueda, Takashi [9 ]
Oiwa, Kazuhiro [4 ]
Akhmanova, Anna [2 ]
Brock, Roland [3 ]
Spang, Anne [10 ]
Friml, Jiri [1 ,11 ,12 ,13 ]
机构
[1] Univ Tubingen, ZMBP, D-72076 Tubingen, Germany
[2] Erasmus MC, Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands
[3] Univ Tubingen, Inst Cell Biol, D-72076 Tubingen, Germany
[4] Nat Inst Informat & Commun Technol, Kansai Adv Res Ctr, Kobe, Hyogo 6512492, Japan
[5] Univ Heidelberg, Heidelberg Inst Plant Sci, D-69120 Heidelberg, Germany
[6] Acad Sci Czech Republic, Inst Microbiol, CR-14220 Prague 4, Czech Republic
[7] Univ Amsterdam, Swammerdam Inst Life Sci, Sect Biol Cytol, NL-1098 SM Amsterdam, Netherlands
[8] Acad Sci Czech Republic, Inst Expt Bot, CR-16502 Prague 6, Czech Republic
[9] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan
[10] Friedrich Miescher Lab Max Planck Soc, D-72076 Tubingen, Germany
[11] Masaryk Univ, Dept Funct Genom, CZ-62500 Brno, Czech Republic
[12] Univ Ghent VIB, Flanders Inst Biotechnol, Dept Plant Syst Biol, B-9052 Ghent, Belgium
[13] Univ Ghent VIB, Dept Mol Genet, B-9052 Ghent, Belgium
[14] Andres Bello Univ, Millennium Nucleus Plant Cell Biol & Ctr Plant Bi, Santiago 8370146, Chile
关键词
PIN proteins; plant development; vesicle traffic; auxin efflux inhibitors;
D O I
10.1073/pnas.0711414105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.
引用
收藏
页码:4489 / 4494
页数:6
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