Polymerase chain reaction for the detection of Phaseolus vulgaris beans in chestnut puree

被引：8

作者：

Krahulcová, J ^{[1
]}

Pangallo, D ^{[1
]}

Piknová, L ^{[1
]}

Siekel, P ^{[1
]}

Kuchta, T ^{[1
]}

机构：

[1] Food Res Inst, Bratislava 82475 26, Slovakia

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2003年 / 217卷 / 01期

关键词：

polymerase chain reaction; Phaseolus vulgaris; chestnut puree; model mixtures;

D O I：

10.1007/s00217-003-0703-6

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A method based upon polymerase chain reaction (PCR) for the detection of Phaseolus vulgaris beans in chestnut puree was developed. The method involves DNA isolation by the GeneSpin kit and PCR with primers oriented to a sequence of a nuclear gene encoding PvLEA-18, a late embryogenesis-abundant protein of beans. The PCR method was shown to be specific for Ph. vulgaris, producing a 100 bp fragment with six Ph. vulgaris cultivars, and negative results with four non-Ph. vulgaris Phaseolus samples and five non-Phaseolus legumes. When evaluated with model mixtures of Ph. vulgaris beans and the chestnut puree, a detection limit of 1% was determined.

引用

页码：80 / 82

页数：3

共 6 条

[1] POLYMERASE CHAIN-REACTION (PCR) - A POSSIBLE ALTERNATIVE TO IMMUNOCHEMICAL METHODS ASSURING SAFETY AND QUALITY OF FOOD - DETECTION OF WHEAT CONTAMINATION IN NON-WHEAT FOOD-PRODUCTS [J].