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The counterion influence on cationic lipid-mediated transfection of plasmid DNA

被引：35
作者：
Aberle, AM
Bennett, MJ
Malone, RW
Nantz, MH
机构：
[1] UNIV CALIF DAVIS,DEPT CHEM,DAVIS,CA 95616
[2] UNIV CALIF DAVIS,DEPT MED PATHOL,DAVIS,CA 95616
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1996年 / 1299卷 / 03期
关键词：
counterion effect; cationic lipid; liposome; transfection; gene transfer; DNA;
D O I：
10.1016/0005-2760(95)00230-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A panel of DOTAP analogs was prepared by altering the anionic counterion that accompanies the trimethylammonium polar domain. The transfection of plasmid DNA into NIH3T3 cells and mouse lung was examined using the counterion analogs. The in vitro transfection activity decreased as follows: DOTAP . bisulfate > trifluoromethanesulfonate similar to iodide similar to bromide > dihydrogenphosphate similar to chloride similar to acetate > sulfate. A similar activity trend was observed in vivo.
引用
收藏
页码：281 / 283
页数：3
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