Nonsense-mutated inlA and prfA not widely distributed in Listeria monocytogenes isolates from ready-to-eat seafood products in Japan

被引：56

作者：

Handa-Miya, Satoko ^{[1
]}

Kimura, Bon ^{[1
]}

Takahashi, Hajime ^{[1
]}

Sato, Miki ^{[1
]}

Ishikawa, Tatsuya ^{[1
]}

Igarashi, Kazunori ^{[1
]}

Fujii, Tateo ^{[1
]}

机构：

[1] Tokyo Univ Marine Sci & Technol, Fac Marine Sci, Dept Food Sci & Technol, Minato Ku, Tokyo 1088577, Japan

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2007年 / 117卷 / 03期

关键词：

Listeria monocytogenes; internalin A; ready-to-cat; seafood;

D O I：

10.1016/j.ijfoodmicro.2007.05.003

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

InIA is a surface protein participating in the entry of Listeria monocytogenes into mammalian non-phagocytic cells. PrfA is a positive regulatory factor that regulates the expression of a set of virulence genes. Recent studies revealed that some L. monocytogenes strains have a truncated form of these proteins because of nonsense mutations in their sequences, and these truncations contribute to the significant reduction in virulence of this pathogen. In this study, sequence analyses of inIA and prfA among L. monocytogenes isolated from ready-to-eat seafood revealed that only one out of 59 isolates had a nonsense-mutated MIA and all had non-mutated prfA. This indicated that these strains could be fully virulent based on the sizes of these proteins. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：312 / 318

页数：7

共 54 条

[1] A 6-HOUR IN-VITRO VIRULENCE ASSAY FOR LISTERIA-MONOCYTOGENES USING MYELOMA AND HYBRIDOMA CELLS FROM MURINE AND HUMAN SOURCES [J].

BHUNIA, AK ;

STEELE, PJ ;

WESTBROOK, DG ;

BLY, LA ;

MALONEY, TP ;

JOHNSON, MG .

MICROBIAL PATHOGENESIS, 1994, 16 (02) :99-110

[2] Variation in biofilm formation among strains of Listeria monocytogenes [J].

Borucki, MK ;

Peppin, JD ;

White, D ;

Loge, F ;

Call, DR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (12) :7336-7342

[3]

Bruce J, 1996, FOOD TECHNOL-CHICAGO, V50, P77

[4] Use of PFGE typing for tracing contamination with Listeria monocytogenes in three cold-smoked salmon processing plants [J].

Dauphin, G ;

Ragimbeau, C ;

Malle, P .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 64 (1-2) :51-61

[5] Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation [J].

Djordjevic, D ;

Wiedmann, M ;

McLandsborough, LA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (06) :2950-2958

[6]

EC, 2005, Commission Regulation (EC) No 2073

[7] Prevalence and characteristics of Shigatoxin-producing Escherichia coli and Listeria monocytogenes strains isolated from minced meat in Switzerland [J].

Fantelli, K ;

Stephan, R .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 70 (1-2) :63-69

[8] Present situation in Canada regarding Listeria monocytogenes and ready-to-eat seafood products [J].

Farber, JM .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2000, 62 (03) :247-251

[9] LISTERIA-MONOCYTOGENES, A FOOD-BORNE PATHOGEN [J].

FARBER, JM ;

PETERKIN, PI .

MICROBIOLOGICAL REVIEWS, 1991, 55 (03) :476-511

[10] ENTRY OF L-MONOCYTOGENES INTO CELLS IS MEDIATED BY INTERNALIN, A REPEAT PROTEIN REMINISCENT OF SURFACE-ANTIGENS FROM GRAM-POSITIVE COCCI [J].

GAILLARD, JL ;

BERCHE, P ;

FREHEL, C ;

GOUIN, E ;

COSSART, P .

CELL, 1991, 65 (07) :1127-1141

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