Comparative sequence analysis of a region on human chromosome 13q14, frequently deleted in B-cell chronic lymphocytic leukemia, and its homologous region on mouse chromosome 14

被引：28

作者：

Kapanadze, B

Makeeva, N

Corcoran, M

Jareborg, N

Hammarsund, M

Baranova, A

Zabarovsky, E

Vorontsova, O

Merup, M

Gahrton, G

Jansson, M

Yankovsky, N

Einhorn, S

Oscier, D

Grandér, D

Sangfelt, O

机构：

[1] Karolinska Hosp, CCK, Radiumhemmets Res Lab, S-17176 Stockholm, Sweden

[2] Royal Bournemouth Hosp, Mol Biol Lab, Bournemouth BH7 7DW, Dorset, England

[3] Russian Acad Sci, Inst Gen Genet, Genome Anal Lab, Moscow 117809, Russia

[4] Karolinska Inst, Ctr Genome Res, S-17177 Stockholm, Sweden

[5] Karolinska Inst, Ctr Microbiol & Tumor Biol, S-17177 Stockholm, Sweden

[6] Huddinge Hosp, Dept Hematol & Med, S-14186 Huddinge, Sweden

来源：

GENOMICS | 2000年 / 70卷 / 03期

关键词：

D O I：

10.1006/geno.2000.6386

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Previous studies have indicated the presence of a putative tumor suppressor gene on human chromosome 13q14, commonly deleted in patients with B-cell chronic lymphocytic leukemia (B-CLL). We have recently identified a minimally deleted region encompassing parts of two adjacent genes, termed LEU1 and LEU2 (leukemia-associated genes 1 and 2), and several additional transcripts. In addition, 50 kb centromeric to this region we have identified another gene, LEU5/RFP2. To elucidate further the complex genomic organization of this region, we have identified, mapped, and sequenced the homologous region in the mouse. Fluorescence in situ hybridization analysis demonstrated that the region maps to mouse chromosome 14. The overall organization and gene order in this region were found to be highly conserved in the mouse. Sequence comparison between the human deletion hotspot region and its homologous mouse region revealed a high degree of sequence conservation with an overall score of 74%. However, our data also show that in terms of transcribed sequences, only two of those, human LEU2 and LEU5/RFP2, are clearly conserved, strengthening the case for these genes as putative candidate B-CLL tumor suppressor genes. (C) 2000 Academic Press.

引用

页码：327 / 334

页数：8

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