The neurons of the ground squirrel retina as revealed by immunostains for calcium binding proteins and neurotransmitters

被引:48
作者
Cuenca, N [1 ]
Deng, P
Linberg, KA
Lewis, GP
Fisher, SK
Kolb, H
机构
[1] Univ Alicante, Dept Biotechnol, E-03080 Alicante, Spain
[2] Univ Utah, Moran Eye Ctr, Dept Ophthalmol, Salt Lake City, UT 84132 USA
[3] Univ Calif Santa Barbara, Neurosci Res Inst, Santa Barbara, CA 93106 USA
来源
JOURNAL OF NEUROCYTOLOGY | 2002年 / 31卷 / 8-9期
关键词
D O I
10.1023/A:1025791512555
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Ground squirrel retinas were immunostained with antibodies against calcium binding proteins (CBPs) and classical neurotransmitters in order to describe neuronal phenotypes in a diurnal mammalian retina and to then compare these neurons with those of more commonly studied nocturnal retinas like cats' and rabbits'. Double immunostained tissue was examined by confocal microscopy using antibodies against the following: rhodopsin and the CBPs, calbindin, calretinin, parvalbumin, calmodulin and recoverin (CB, CR, PV, CM, RV), glycine, GABA, choline acetyltransferase (CHAT) and tyrosine hydroxylase (TOH). In ground squirrel retina, the traditional cholinergic mirror symmetric amacrine cells colocalize CHAT with PV and GABA and faintly with glycine. A second cholinergic amacrine cell type colocalizes glycine alone. CR is found in at least 3 different amacrine cell types. The CR-immunoreactive (IR) cell population is a mixture of glycinergic and GABAergic types. The dopamine cell type IR to tyrosine hydroxylase has the typical morphology of a wide field cell with dendrites in S1 but the "rings'' seen in cat or rabbit retina are not as numerous. TOH-IR amacrine cells send large club-shaped processes to the outer plexiform layer. CB and CR are in bipolar cells, A- and B-type horizontal cells and several amacrine cell types. Anti-rhodopsin labels the low density rod photoreceptor population in this species. Anti-recoverin labels cones and some bipolar cells while PKC is found in several different bipolar cell types. One ganglion cell with dendritic branching in S3 is strongly CR-IR. We find no evidence for an AII amacrine cell in the ground squirrel, with either anti-CR or anti-PV. An amacrine cell with similarity to the DAP1-3 cell of rabbit is CR-IR and glycine-IR. We discuss this labeling pattern in relationship to other mammalian species. The differences in staining patterns and phenotypes revealed suggest a functional diversity in the populations of amacrine cells according to whether the retinas are rod or cone dominated.
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页码:649 / 666
页数:18
相关论文
共 72 条
[1]   CHARACTERIZATION OF THE COLOR RELATED RECEPTOR MOSAIC IN THE GROUND-SQUIRREL RETINA [J].
AHNELT, PK .
VISION RESEARCH, 1985, 25 (11) :1557-1567
[2]   MORPHOLOGIES OF RABBIT RETINAL GANGLION-CELLS WITH CONCENTRIC RECEPTIVE-FIELDS [J].
AMTHOR, FR ;
TAKAHASHI, ES ;
OYSTER, CW .
JOURNAL OF COMPARATIVE NEUROLOGY, 1989, 280 (01) :72-96
[3]   MORPHOLOGIES OF RABBIT RETINAL GANGLION-CELLS WITH COMPLEX RECEPTIVE-FIELDS [J].
AMTHOR, FR ;
TAKAHASHI, ES ;
OYSTER, CW .
JOURNAL OF COMPARATIVE NEUROLOGY, 1989, 280 (01) :97-121
[4]   THE DISTRIBUTION OF ENZYMES WHICH SYNTHESIZE NICOTINAMIDE ADENINE-DINUCLEOTIDE AND NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE IN MONKEY, RABBIT, AND GROUND-SQUIRREL RETINAS [J].
BERGER, SJ ;
DEVRIES, GW .
JOURNAL OF NEUROCHEMISTRY, 1982, 38 (03) :821-826
[5]  
BLOOMFIELD SA, 2001, BRAIN RES REV, P185
[6]   AII AMACRINE CELL-POPULATION IN THE RABBIT RETINA - IDENTIFICATION BY PARVALBUMIN IMMUNOREACTIVITY [J].
CASINI, G ;
RICKMAN, DW ;
BRECHA, NC .
JOURNAL OF COMPARATIVE NEUROLOGY, 1995, 356 (01) :132-142
[7]   Choline acetyltransferase is found in terminals of horizontal cells that label with GABA, nitric oxide synthase and calcium binding proteins in the turtle retina [J].
Cuenca, N ;
Haverkamp, S ;
Kolb, H .
BRAIN RESEARCH, 2000, 878 (1-2) :228-239
[8]   Choline acetyltransferase is expressed by non-starburst amacrine cells in the ground squirrel retina [J].
Cuenca, N ;
Deng, P ;
Linberg, KA ;
Fisher, SK ;
Kolb, H .
BRAIN RESEARCH, 2003, 964 (01) :21-30
[9]   Vesicular γ-aminobutyric acid transporter expression in amacrine and horizontal cells [J].
Cueva, JG ;
Haverkamp, S ;
Reimer, RJ ;
Edwards, R ;
Wässle, H ;
Brecha, NC .
JOURNAL OF COMPARATIVE NEUROLOGY, 2002, 445 (03) :227-237
[10]   Localization of neurotransmitters and calcium binding proteins to neurons of salamander and mudpuppy retinas [J].
Deng, P ;
Cuenca, N ;
Doerr, T ;
Pow, DV ;
Miller, R ;
Kolb, H .
VISION RESEARCH, 2001, 41 (14) :1771-1783