Human antibodies against Plasmodium falciparum liver-stage antigen 3 cross-react with Plasmodium yoelii preerythrocytic-stage epitopes and inhibit sporozoite invasion in vitro and in vivo

被引:27
作者
Brahimi, K [1 ]
Badell, E [1 ]
Sauzet, JP [1 ]
BenMohamed, L [1 ]
Daubersies, P [1 ]
Guérin-Marchand, C [1 ]
Snounou, G [1 ]
Druilhe, P [1 ]
机构
[1] Inst Pasteur, Lab Parasitol Biomed, F-75015 Paris 15, France
关键词
D O I
10.1128/IAI.69.6.3845-3952.2001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The Plasmodium falciparum liver-stage antigen 3 (LSA3), a recently identified preerythrocytic antigen, induces protection against malaria in chimpanzees. Using antibodies from individuals with hyperimmunity to malaria affinity purified on recombinant or synthetic polypeptides of LSA3, we identified four non-crossreactive B-cell epitopes in Plasmodium yoelii preerythrocytic stages. On sporozoites the P. yoelii protein detected has a molecular mass similar to that of LSA3. T-cell epitopes cross-reacting with P. yoelii were also demonstrated using peripheral blood lymphocytes from LSA3-immunized chimpanzees. In contrast, no cross-reactive epitopes were found in Plasmodium berghei. LSA3-specific human antibodies exerted up to 100% inhibition of in vitro invasion of P. yoelii sporozoites into mouse hepatocytes. This strong in vitro activity was reproduced in vivo by passive transfer of LSA3 antibodies. These results indicate that the homologous epitopes may be biologically functional and suggest that P. yoelii could be used as a model to assess the antisporozoite activity of anti-LSA3 antibodies.
引用
收藏
页码:3845 / 3852
页数:8
相关论文
共 33 条
[1]   High immunogenicity in chimpanzees of peptides and lipopeptides derived from four new Plasmodium falciparum pre-erythrocytic molecules [J].
Benmohamed, L ;
Thomas, A ;
Bossus, M ;
Brahimi, K ;
Wubben, J ;
Gras-Masse, H ;
Druilhe, P .
VACCINE, 2000, 18 (25) :2843-2855
[2]   Lipopeptide immunization without adjuvant induces potent and long-lasting B, T helper, and cytotoxic T lymphocyte responses against a malaria liver stage antigen in mice and chimpanzees [J].
BenMohamed, L ;
GrasMasse, H ;
Tartar, A ;
Daubersies, P ;
Brahimi, K ;
Bossus, M ;
Thomas, A ;
Druilhe, P .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1997, 27 (05) :1242-1253
[3]  
Bottius E, 1996, J IMMUNOL, V156, P2874
[4]   FAST IMMUNOPURIFICATION OF SMALL AMOUNTS OF SPECIFIC ANTIBODIES ON PEPTIDES BOUND TO ELISA PLATES [J].
BRAHIMI, K ;
PERIGNON, JL ;
BOSSUS, M ;
GRAS, H ;
TARTAR, A ;
DRUILHE, P .
JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 162 (01) :69-75
[5]  
BURNS JM, 1989, J IMMUNOL, V142, P2835
[6]   PLASMODIUM-YOELII - 17-KDA HEPATIC AND ERYTHROCYTIC STAGE PROTEIN IS THE TARGET OF AN INHIBITORY MONOCLONAL-ANTIBODY [J].
CHAROENVIT, Y ;
MELLOUK, S ;
SEDEGAH, M ;
TOYOSHIMA, T ;
LEEF, MF ;
DELAVEGA, P ;
BEAUDOIN, RL ;
AIKAWA, M ;
FALLARME, V ;
HOFFMAN, SL .
EXPERIMENTAL PARASITOLOGY, 1995, 80 (03) :419-429
[7]   INABILITY OF MALARIA VACCINE TO INDUCE ANTIBODIES TO A PROTECTIVE EPITOPE WITHIN ITS SEQUENCE [J].
CHAROENVIT, Y ;
COLLINS, WE ;
JONES, TR ;
MILLET, P ;
YUAN, L ;
CAMPBELL, GH ;
BEAUDOIN, RL ;
BRODERSON, JR ;
HOFFMAN, SL .
SCIENCE, 1991, 251 (4994) :668-671
[8]  
CHAROENVIT Y, 1991, J IMMUNOL, V146, P1020
[9]   IMMUNIZATION OF MAN AGAINST SPOROZITE-INDUCED FALCIPARUM MALARIA [J].
CLYDE, DF ;
MOST, H ;
MCCARTHY, VC ;
VANDERBERG, JP .
AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 1973, 266 (03) :169-177
[10]  
DASILVEIRA FJ, 1984, MOL BIOCHEM PARASIT, V8, P45