Molecular epidemiology of Mycobacterium bovis isolates from South America

被引:8
作者
Fisanotti, JC
Alito, A
Bigi, F
Latini, O
Roxo, E
Cicuta, E
Zumarraga, MJ
Cataldi, A
Romano, MI
机构
[1] Inst Nacl Tecnol Agropecuaria, Ctr Invest Ciencias Vet, Inst Biotecnol, RA-1708 Moron, Buenos Aires, Argentina
[2] Inst Nacl Epidemiol, Santa Fe, Argentina
[3] Inst Biol, Sao Paulo, Brazil
[4] Univ Nacl Noreste, Fac Ciencias Vet, Corrientes, Argentina
关键词
Mycobacterium bovis; DNA fingerprinting; DR/PGRS markers;
D O I
10.1016/S0378-1135(98)00150-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
One hundred seventy-eight isolates of Mycobacterium bovis were subjected to DNA restriction fragment length polymorphism (RFLP) analysis, using the direct repeat element (DR) and the polymorphic CC-rich repeat sequence (PGRS) as probes. By combining the patterns generated by the two repeat DNA elements, 93 different patterns were observed. One hundred-one isolates were grouped in clusters, which include 25 different clusters. One pattern was the most frequently observed, clustering 18.5% of isolates. It was only found in the Center and northeast regions of Argentina and in one isolate from Paraguay. The isolates from Brazil analyzed here presented exclusive patterns (only found in a particular region). The number of exclusive patterns was high in all Argentine regions: northeast 78%, center 81%, and Buenos Aires 81%. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:251 / 257
页数:7
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