Modified peptidoglycan chemical composition in shape-altered Escherichia coli

被引:16
作者
Signoretto, C
DiStefano, F
Canepari, P
机构
[1] Istituto di Microbiologia, Università di Verona, 37134 Verona, Strada Le Grazie
来源
MICROBIOLOGY-UK | 1996年 / 142卷
关键词
Escherichia coli; peptidoglycan; bacterial shape; cell division;
D O I
10.1099/13500872-142-8-1919
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Peptidoglycan synthesis and its fine chemical composition were studied in dividing cocci of Escherichia coli carrying the lov-1 mutation and in which the coccal shape was obtained either by mecillinam treatment or by transferring a pbpA mutation (penicillin-binding protein 2(-) phenotype), as compared to normal rods and non-dividing cocci. Synchronously dividing cocci showed peptidoglycan synthesis only in the cell cycle phase corresponding to cell septation. During the phase corresponding to lateral wall elongation, peptidoglycan synthesis was strongly reduced. This type of synthesis suggests that the dividing cocci consisted only of the two poles. Analysis of the muropeptide composition revealed a specific fourfold increase in the tetra-tetra-tetra trimer in dividing cocci as compared to non-dividing cocci or parental rods. We postulate that, in E. coli, the chemical composition of septal peptidoglycan differs from that of lateral wall peptidoglycan.
引用
收藏
页码:1919 / 1926
页数:8
相关论文
共 27 条
[1]   PEPTIDOGLYCAN BIOSYNTHESIS IN STATIONARY-PHASE CELLS OF ESCHERICHIA-COLI [J].
BLASCO, B ;
PISABARRO, AG ;
DEPEDRO, MA .
JOURNAL OF BACTERIOLOGY, 1988, 170 (11) :5224-5228
[2]  
BOULOC P, 1988, REV INFECT DIS, V10, P905
[3]   THE ESCHERICHIA-COLI LOV GENE-PRODUCT CONNECTS PEPTIDOGLYCAN SYNTHESIS, RIBOSOMES AND GROWTH-RATE [J].
BOULOC, P ;
JAFFE, A ;
DARI, R .
EMBO JOURNAL, 1989, 8 (01) :317-323
[4]  
CANEPARI P, 1993, MICROBIOLOGICA, V16, P165
[5]  
DANEOMOORE L, 1977, CELL SURF REV, V4, P597
[6]   PEPTIDOGLYCAN SYNTHESIS DURING THE CELL-CYCLE OF ESCHERICHIA-COLI - COMPOSITION AND MODE OF INSERTION [J].
DEJONGE, BLM ;
WIENTJES, FB ;
JURIDA, I ;
DRIEHUIS, F ;
WOUTERS, JTM ;
NANNINGA, N .
JOURNAL OF BACTERIOLOGY, 1989, 171 (11) :5783-5794
[7]   EFFECT OF GROWTH-RATE AND CELL-SHAPE ON THE PEPTIDOGLYCAN COMPOSITION IN ESCHERICHIA-COLI [J].
DRIEHUIS, F ;
WOUTERS, JTM .
JOURNAL OF BACTERIOLOGY, 1987, 169 (01) :97-101
[8]  
GLAUNER B, 1990, J BIOL CHEM, V265, P18988
[10]  
GLAUNER B, 1988, J BIOL CHEM, V263, P10088