Techniques to measure lipase and esterase activity in vitro

被引：169

作者：

Gilham, D

Lehner, R

机构：

[1] Univ Alberta, Dept Cell Biol, CIHR Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada

[2] Univ Alberta, Dept Pediat, CIHR Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada

来源：

METHODS | 2005年 / 36卷 / 02期

关键词：

lipase; esterase; lipid; triacylglycerol; lipolysis; fatty acid;

D O I：

10.1016/j.ymeth.2004.11.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipases and esterases constitute a large category of enzymes. They are ubiquitous in nature, found in bacteria, fungi, and animals. The family members address a wide variety of structurally diverse substrates. Appropriately, a large number of assays have been developed to analyze their activity in vitro. Here, we present an overview of these enzymes, along with protocols for common assays performed in solution. An emphasis is placed on assays for enzymes that can hydrolyze triacylglycerols. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：139 / 147

页数：9

共 95 条

[1] Heterologous expression, purification, and characterization of human triacylglycerol hydrolase [J].